Basic Research
Copyright ©2006 Baishideng Publishing Group Co.
World J Gastroenterol. Feb 21, 2006; 12(7): 1063-1070
Published online Feb 21, 2006. doi: 10.3748/wjg.v12.i7.1063
Table 1 Primers for reverse-transcription-PCR of HGF, TGF-α, p21, p27 and p53 mRNAs and 28S rRNA.
Target sequence1PrimerSequence (5’- 3’)
28S28S ForwardGGC CAA GCG TTC ATA GCG AC
28S ReverseGAG GCG TTC AGT CAT AAT CC
HGF[38]HGF ForwardCCC GGT GCT GCA GCA TGT CCT
HGF ReverseTCC CCT CGA TTT CGA CAG
TGF-α[38]TGF-α ForwardGAC AAG TTG AAC AAG AAC CTC
TGF-α ReverseCGT CAT CCA CCT AAT ACA TAA G
p21[52]p21 ForwardATG TCC GAT CCT GGT GAT GTC
p21 ReverseCAC TTC AGG GCT TTC TCT TGC
p27[52]p27 ForwardGCA GCT TGC CCG AGT TCT AC
p27 ReverseTTC TTG GGC GTC TGC TCC AC
p53[53]p53 ForwardTCC TCC CCA ACA TCT TAT CC
p53 ReverseGCA CAA ACA CGA ACC TCA AA