Immunoproteome analysis of soluble and membrane proteins of Shigella flexneri 2457T

Table 1 Proteins identified from the soluble and membrane protein immunoblots

SpotNo.	Accession No. (GenBank)	Name	Description	Obs. pI/Mr1	Exp. pI/Mr2	Coverage (%)	n (peptidesmatched)
1	GI:30042642	TolC	Outer membrane channel	5.10/65.0	5.35/53.8	17	7
2	GI:38000008	OmpA	Outer membrane protein A (fragment)	5.00-5.50/42.0	5.47/35.3	38	8
3	GI:13310620	IpaD	37 K membrane antigen	5.80/40.0	5.55/36.6	39	9
4	GI:30042535	AnsB	Periplasmic L-asparaginase	6.10/38.0	5.95/36.8	22	6
5	GI:17380384	GroEL	60 kDa chaperonin, protein Cpn60	4.80/68.0	4.85/57.3	18	7
6	GI:32307045	Spa33	Surface presentation of proteins antigen	5.80/68.0	5.67/33.4	23	4
7	GI:30065275	Ggt	Gamma-glutamyltranspeptidase	5.00/65.0	5.55/61.8	15	4
8	GI:30040341	TolB	Involved in the tonB-independent uptake of group A colicins	6.50/46.0	7.0/46.0	18	6

¹The observed pI and molecular weight of protein calculated from the protein spots position on the CBB G250 2D gel;

²The predicted pI and molecular weight of the protein calculated from MSDB database.