Enhanced expression of epidermal growth factor receptor gene in gastric mucosal cells by the serum derived from rats treated with electroacupuncture at stomach meridian acupoints

Table 1 The primer sequences and sizes of amplification products

EGFR	Forward primer:	664 bp
	5'-AGT GGT CCT TGG AAA CTT GG-3'
	Reverse primer:
	5'- GTT GAC ATC CAT CTG GTA CG-3'
GADPH	Forward primer:	426 bp
	5'-TGC TGA GTA TGT CGT GGA GTC -3'
	Reverse primer:
	5'-AAG GCC ATG CCA GTG AGC TTC -3'

Table 2 The EGFR gene expression in gastric mucosal cells (mean ± SD, n = 8)

Group	EGFR mRNA/GAPDH mRNA
Normal group	0.6860 ± 0.0594
Model group	0.7104 ± 0.0457d
Model serum group	0.8516 ± 0.0409bd
Stomach serum group	1.2272 ± 0.0813b
Gallbladder serum group	0.9640 ± 0.0387bd

^bP < 0.01 vs Model group;

^dP < 0.01 vs Stomach serum group.