Virological course of hepatitis A virus as determined by real time RT-PCR: Correlation with biochemical, immunological and genotypic profiles

Table 1 Clinical, biochemical and immunological characterization of different groups (AVH, s-AH, and normal control)

Characteristic	AVH1	s-AH2	Normal Control3
No. of cases	07	03	10
Sex (M/F)	3/4	3/0	7/3
Age (mean ± SD)	0.4 ± 17.9	27.7 ± 2.08	26.2 ± 3.6
ALT (mean ± SD; IU/L)	1070.9 ± 894.3	1713.9 ± 886.3	23.6 ± 7.2
AST (mean ± SD; IU/L)	621.3 ± 242.8	1614 ± 234.7	23.8 ± 5.8
T. Bilirubin (mean ± SD; mg/dL)	9.8 ± 3.8	24.5 ± 3.0	0.74 ± 0.43
Prothrombin Time (Seconds)	14.3 ± 1.1	21.0 ± 2.0	12.5 ± 0.5
Mean CD4+/CD8+	1.17 ± 0.11	0.83 ± 0.12	1.52 ± 0.11

ALT: AVH vs s-AH P = 0.29 (not significant); AVH vs NC P = 0.0014 (highly significant); s-AH vs NC P = 0.001 (highly significant); AST: AVH vs s-AH P = 0.0034 (highly significant); AVH vs NC P = 0.00014 (highly significant); s-AH vs NC P = 0.00044 (highly significant); T. Bil: AVH vs s-AH P = 0.076 (significant); AVH vs NC P = 0.00017 (highly significant); s-AH vs NC P = 0.0023 (highly significant); PT: AVH vs s-AH P = 0.11 (not significant); AVH vs NC P = 0.44 (not significant); s-AH vs NC P = 0.02 (significant); Mean CD4⁺/CD8⁺: AVH vs s-AH P = 0.46 (not significant); AVH vs NC P = 0.22 (not significant); s-AH vs NC P = 0.0002 (highly significant). Note: AVH¹ (Acute viral hepatitis); s-AH² (Severe acute hepatitis); NC³ (Normal Control).

Table 2 Comparison of patient’s genotypes to viral load and geographical distribution

Patients	Genotype	Maximal viral load	Geographical
		Copies/mL	distribution
Ind-301	IA	4.5 × 105	New Delhi
1Ind-302	IA	6.0 × 105	New Delhi
Ind-303	IIIA	2.6 × 105	Uttar Pradesh
Ind-304	IIIA	1.0 × 105	Delhi
1Ind-305	IIIA	5.0 × 105	Delhi
Ind-306	IIIA	1.2 × 105	New Delhi
1Ind-307	IIIA	4.6 × 105	Uttar Pradesh
Ind-308	IIIA	4.1 × 103	New Delhi
Ind-309	IIIA	3.4 × 104	Haryana
Ind-310	IIIA	9.8 × 104	New Delhi

¹Represents severe acute hepatitis.