Role of N-acetylcysteine in rifampicin-induced hepatic injury of young rats

Table 1 Effect of RMP treatment (50 mg/kg/day for 3 wk) and NAC (100 mg/kg/day for 3 wk) on total, protein-bound and non-protein thiols in rats (µmole /g liver) (mean±SD)

Group	Total thiols	Protein bound	Non protein thiols(GSH)
Control	13.42 ± 0.52	10.09 ± 0.45	3.23 ± 0.42
RMP	16.77 ± 0.61a	13.49 ± 0.42a	2.98 ± 0.61
RMP + NAC	17.17 ± 0.78b	13.92 ± 0.51b	3.37 ± 0.72
NAC	13.44 ± 0.50	9.55 ± 0.25	3.42 ± 0.43

^aP < 0.05 vs control, ^bP < 0.001 vs control.

Table 2 Effect of RMP treatment (50 mg/kg/day for 3 wk) and NAC (100 mg/kg/day for 3 wk) on lipid peroxidation, catalase, superoxide dismutase, glutathione-S-transferase(nmol/min/mg protein) in rats (mean±SD)

Group	Lipid peroxidation (nmolMDA/g tissue/10min)	Catalase(µmol/min/mgprotein)	Superoxidedismutase (IU)	Glutathione-S-transferaseusing EA
Control	120.47±9.3	310.32±31.2	17.92±2.25	13.94±2.27
RMP	163.52±22.6b	204.92±28.6b	11.71±1.39b	18.58±1.52Wb
RMP + NAC	138.71±13.2Wa	269.48±19.5Wa	14.38±2.47Wa	15.92±1.38a
NAC	103.59±12.9	317.51±38.7a	16.47±2.80	14.59±2.18

^aP < 0.05 vs control, ^bP < 0.001 vs control.

Table 3 Effect of RMP treatment (50 mg/kg/day for 3 wk) and NAC (100 mg/kg/day for 3 wk) on glutathione peroxidase, glutathione reductase and cytochrome P₄₅₀ (nmol/min/mg protein) (mean±SD)

Group	Glutathioneperoxidase	Glutathionereductase	Cytochrome P450
Control (C)	458.37±45.19	25.37±3.82	0.431±0.050
RMP	287.18±39.82Wb	27.01±2.75	0.540±0.039b
RMP + NAC	385.99±50.47a	27.47±3.27	0.509±0.042a
NAC	474.07±37.92	26.93±4.01	0.412±0.047

^aP < 0.05 vs control, ^bP < 0.001 vs control.