Prognostic values of tumor endothelial markers in patients with colorectal cancer

Table 1 Primer sequences for conventional PCR.

Primers	Sense primer (5’-3’)	Antisense primer (5’-3’)
TEM-1	gtggcttcgagtgttattg	gaagagctccggatatttg
TEM-2	agccatgatgaagactttgt	cttgaggtcactgttgacg
TEM-6	acccgtgacgtcattttc	tgtacttgcttcgagcatc
TEM-7	ggagcaggtcacgatgag	gtgaaactgcccttgtctt
TEM-7R	cttgattggcagtatggagt	gagatgtacatggtcccact
TEM-8	catttcaagttgtcgtgaga	gacgcatattgttgttgaga

Table 2 Primer sequences for quantitative PCR.

Molecules	Sense primer (5’-3’)	Z primer (5’-3’)
TEM-1	cttgcccactgggatgat	Actgaacgtgaccgtacaacctatgaatcctctgatgg
TEM-2	agtctcaccttgagtgtggt	Actgaacctgaccgtacactcctccacagcatctctta
TEM-6	acccgtgaggtcattttc	Actgaacctgaccgtacattcaaccttcccatagtcag
TEM-7	agaacgaccacatcacctt	Actgaacctgaccgtacatggagagagttggagtcaa
TEM-7R	cttgattggcagtatggagt	Actgaacctgaccgtacagtctaccgccttgagaaag
TEM-8	acagggtcctctgcagctt	Actgaacctgaccgtacactttcatgccaacttgttt

Table 3 Expression of TEMs in colon tissues (percentage positive), using conventional PCR.

	Normal tissues (%)	Tumor tissues (%)	P
TEM-1	38	95.5	<0.01
TEM-2	45	58.3	>0.05
TEM-6	35	56	<0.04
TEM-7	15	77.5	<0.04
TEM-7R	12.5	79.5	<0.03
TEM-8	1	85	<0.001