Basic Research
Copyright ©2005 Baishideng Publishing Group Inc.
World J Gastroenterol. Feb 14, 2005; 11(6): 797-802
Published online Feb 14, 2005. doi: 10.3748/wjg.v11.i6.797
Table 1 Primers used for PCR and sequencing of UGT1A7 gene.
RegionPrimer
Target size (bp)
NameSequence
PromoterPCR:U7F15’TGAATGAATAAGTACACGCC3’439
U7R15’ATAGAGAAAATGCACTTCGC3’
Sequencing:U7F15’TGAATGAATAAGTACACGCC3’
Exon 1PCR:U7F15’TGAATGAATAAGTACACGCC3’779
U7R25’TAGGGGCAAAATAAATGTTC3’
Sequencing:U7R25’TAGGGGCAAAATAAATGTTC3’
PCR:U7F25’ATACACTCTGGAGGATCAGG3’774
U7R35’GCTACCCAACAATTAAGTGA3’
Sequencing:U7F35’TGTCCCCAGACTTCTCTTAG3’
Table 2 Frequencies of UGT1A7 genotypes in 112 Taiwan Chinese.
UGTIA7Number (%)
*1/*136 (32.1)
*1/*240 (35.7)
*1/*317 (15.2)
*2/*25 (4.5)
*2/*311 (9.8)
*3/*33 (2.7)
Table 3 Allele frequencies of UGT1A7 gene among different ethnic groups.
UGT1A7 allelesTaiwan Chinese (224 chromosomes)Caucasians (288 chromosomes)Japanese (206 chromosomes)P value (by χ2 test)
UGT1A7*10.5760.3580.593<0.001
0.73
UGT1A7*20.2720.2640.1530.93
0.004
UGT1A7*30.1520.3610.255<0.001
0.008
Table 4 Allele frequency of UGT1A7*3 in subjects carrying different UGT1A1 genotypes.
UGT1A1 geneNumberUGT1A7 gene
Allele frequency of UGT1A7*3Relative risk(95% CI)
*1/*1*1/*2*1/*3*2/*2*2/*3*3/*3
Wild type246114108915000.0181
A(TA)6TAA/A(TA)7TAA38131271500.1588.78b
(2.70-28.54)
A(TA)7TAA/A(TA)7TAA3113591030.24213.44b
(4.41-40.99)
211G/211A906104802240.43324.06b
(9.31-62.31)
211A/211A100001600840.9251.11b
(20.24-128.80)
Table 5 Natural or mutagenesis primers, restriction enzymes and the results for UGT1A7 variations.
Position (cDNA)PrimersSequenceRestriction enzymeResult (bp)
-57 T→GU7F15’ TGAATGAATAAGTACACGCCHpyCH4IVN2439
U7R15’ ATAGAGAAAATGCACTTCGC3’V357+382
33 C→A1stPCR
U7F15’ TGAATGAATAAGTACACGCC
U7R25’ TAGGGGCAAAAAATGTTC3’
2ndPCR
U7-33F15’ GGGTGGACTGGCCTCCTG3’1Taq IN115
U7-33R5’ ACTGCATGGTGAACCAGTCGV19+96
387 T→G1stPCR
U7F15’ TGAATGAATAAGTACACGCC
U7R25’ TAGGGGCAAAATAAATGTTC
2ndPCR
U7-387F5’ AAATTGCAGGAGTTCTTA3’1Afl IIN159
U7-387R5’ TGGCAAAATATTCCCCGC3’V140+19
622 T→CU7F35’ TGTCCCCAGACTTCTCTTAGRsa IN447
U7R35’ GCTACCCAACAATTAAGTGAV54+393