Increased expression of mitogen-activated protein kinase and its upstream regulating signal in human gastric cancer

Table 1 Integral optical density value of gastric cancer and adjacent normal mucosa (mean±SD).

Items	n	IOD value		t	P
		Gastric cancer	Normal mucosa
ERK-1	42	159526±65760	122807±65515	3.658	0.001
ERK-2	42	168471±95051	120469±72874	4.758	<0.001
ERK-3	42	118651±71513	70934±68058	5.065	<0.001
P38	30	104776±51650	82930±40392	2.064	0.048
MEK-1	30	116486±45725	101434±49387	2.16	0.027

Table 2 Relationship between ERK1/2 expression and clinicopathological characteristics in poorly differentiated gastric cancers (mean±SD).

Items	n	ERK1			ERK2
		T:N ratio1	F (t)	P	T:N ratio1	F (t)	P
TNM stage
I, II	11	1.01±0.33	2.505	0.022	1.24±0.40	2.297	0.03
III, IV	22	2.64±3.01			2.05±1.54
Lymph node involvement
+	24	2.49±2.91	2.421	0.023	1.98±1.49	2.191	0.036
-	9	1.03±0.36			1.24±0.44
Serosa invasion
+	26	2.39±2.82	2.428	0.022	1.95±1.44	2.585	0.015
-	7	1.01±0.35			1.14±0.36
Tumor size
<5 cm	19	1.97±2.30	0.323	0.749	1.72±1.31	0.309	0.76
>5 cm	14	2.27±2.96			1.86±1.39
Borrmann classification
I	5	1.27±0.53	1.42	0.257	0.96±0.22	1.169	0.338
II	22	2.17±1.64			2.96±3.27
III	12	1.30±0.68			1.02±0.37
IV	3	1.38±0.19			1.59±0.20

¹Average ratio of IOD_tumor: IOD_normals.

Table 3 Expression of ERK-1, ERK-2, ERK-3, p38 and MEK-1 in metastatic lymph nodes (mean±SD).

Items	n	IOD value	t	P
ERK-1
Metastatic lymph nodes	14	195734.7±70508.8
Primary site		210423.7±74207.3	0.608	0.554b
Normal mucosa		155862.3±79052.8	1.453	0.170a
ERK-2
Metastatic lymph nodes	14	186959.5±80801.8
Primary site		211155.1±64116.8	0.893	0.388b
Normal mucosa		127092.4±56585.6	2.579	0.023a
ERK-3
Metastatic lymph nodes	14	140323.2±120532.0
Primary site		98732.1±54021.3	1.261	0.230b
Normal mucosa		50032.9±58937.8	3.116	<0.001a
P38
Metastatic lymph nodes	12	96866.8±76857.3
Primary site		99740.6±38279.6	0.202	0.844b
Normal mucosa		77784.4±30367.0	0.797	0.442a
MEK-1
Metastatic lymph nodes	12	133353.9±42374.3
Primary site		101112.5±39124.0	2.203	0.05b
Normal mucosa		77431.9±31282.1	4.235	<0.001a

^aP<0.05 vs normal mucosa;

^bP>0.05 vs primary site.