Persistence of high CD40 and CD40L expression after restorative proctocolectomy for ulcerative colitis

doi:10.3748/wjg.v11.i34.5303

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Sep 14, 2005 (publication date) through Apr 25, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Clinical Research

World J Gastroenterol. Sep 14, 2005; 11(34): 5303-5308
Published online Sep 14, 2005. doi: 10.3748/wjg.v11.i34.5303

Table 1 Antibodies and sources for flow cytometry

Cluster differentiation	Clone	Isotype	Fluorochromes	Excitation	Emission	Source
CD45	J.33	IgG1	ECD	486-575	610-635	Coulter
CD20	H299 (B1)	IgG2a	FITC	468-505	504-541	Coulter
CD14	M5E2	IgG2a	PE	486-575	568-590	Becton-Dickinson
CD40	5C3	IgG1	PC5	486-580	660-680	Becton-Dickinson
CD154	TRAP1	IgG1	PC5	486-580	660-680	Becton-Dickinson
CD3	UCHT1	IgG1	FITC	468-505	504-541	Caltag

Table 2 CD40 and CD154 expression by lymphocytes in the intestinal mucosa

	Controls	Ileum UC	Pouch	Pouchitis	Healthy pouch
CD40 (%)	4.0 ± 1.62	9.60 ± 2.73	14.0 ± 1.72^a	19.7 ± 2.75^a^c	8.6 ± 1.76^a
CD40L (%)	0.4 ± 0.21	26.0 ± 6.12^a^c	17.5 ± 2^a	20.4 ± 2.54^a^c	12.3 ± 2.64^a

^aP < 0.05 vs controls;

^cP < 0.05 vs healthy pouch.

Table 3 Flow cytometry results: CD40 expression on B cells and monocytes

	HfiCD40+CD20+ (%)	P	HfiCD40+CD14+ (%)	P
UC vs controls	9.7 ± 1.34 vs 3.6 ± 0.99	< 0.05	0.38 ± 0.13 vs 0.09 ± 0.06	NS
Pouch vs controls	9.0 ± 2.37 vs 3.6 ± 0.99	< 0.05	0.16 ± 0.06 vs 0.09 ± 0.06	NS
Extraint+ vs extraint-	16.86 ± 5.25 vs 5.30 ± 1.58	< 0.05	0.1 ± 0.03 vs 0.2 ± 0.13	NS
PDAI ≥ 7 vs PDAI < 7	11.00 ± 3.6 vs 6.40 ± 2.0	NS	0.1 ± 0.04 vs 0.2 ± 0.08	NS
CRP+ vs CRP–	8.6 ± 2.25 vs 10.7 ± 2.0	NS	0.2 ± 0.13 vs 0.7 ± 0.4	NS
ESR+ vs ESR–	8.6 ± 1.8 vs 10.4 ± 2.8	NS	0.3 ± 0.2 vs 0.7 ± 0.4	NS

CRP, C reactive protein; ESR, erythrocyte sedimentation rate; extraint, extraintestinal diseases; Hfi, high fluorescence intensity; NS, non significant; PDAI, pouchitis disease activity index.

Citation: Polese L, Angriman I, Franchis GD, Cecchetto A, Sturniolo GC, D’Incà R, Scarpa M, Ruffolo C, Norberto L, Frego M, D’Amico DF. Persistence of high CD40 and CD40L expression after restorative proctocolectomy for ulcerative colitis. World J Gastroenterol 2005; 11(34): 5303-5308
URL: https://www.wjgnet.com/1007-9327/full/v11/i34/5303.htm
DOI: https://dx.doi.org/10.3748/wjg.v11.i34.5303