Amygdalin inhibits genes related to cell cycle in SNU-C4 human colon cancer cells

Table 1 Sequences of the primers used in RT-PCR analysis

Primer name	Primer sequence (sense/anti-sense)	Fragment length (bp)	Annealing temperature (°C)
EXO1	5’-CTCTTTTGAGAGCAGCAAAT-3’	400	58
	5’-GGTCTGGTCACTTTGACTGTC-3’
ABCF2	5’-TGCACAACAAGAAACTGAAG-3’	523	58
	5’-TGCTCTTGTAAATGCTGATGGT-3’
MRE11A	5’-GAAGGTACGTCGTTTCAGAGAA-3’	456	58
	5’-CGATCTTGACTCTGGGACATGATT-3’
TOP1	5’-CTGTGAAGAGGAACAGTGTGGT-3’	561	58
	5’-GAACAGGTGTCTGAACCAAAAC-3’
FRAP1	5’-GCTCGTAGTTGGGATAACAG-3’	426	58
	5’-GTTGCCAGGACATTATTGAT-3’
EGFR	5’-ATGAAGAAGACATGGACGAC-3’	522	56
	5’-AGAAGTCCTGCTGGTAGTCA-3’
CYCLOPHILIN	5’-ACCCCACCGTGTTCTTCGAC-3’	300	56
	5’-CATTTGCCATGGACAAGATG-3’

Table 2 List of genes downregulated by amygdalin treatment

Gene	Chromosome	Title	Global M
Cell cycle
FRAP1	1p36.2	FK506 binding protein 12-rapamycin associated protein 1	–1.029
TOP1	20q12–q13.1	Topoisomerase (DNA) I	–1.118
MRE11A	11q21	MRE11 meiotic recombination 11 homolog A (S. cerevisiae)	–1.235
EXO1	1q42–q43	Exonuclease 1	–1.740
ABCF2	7q36	ABC, sub-family F (GCN20), member 2	–1.993
Cell growth
FRAP1	1p36.2	FK506 binding protein 12-rapamycin associated protein 1	–1.029
TIEG2	2p25	TGFB inducible early growth response 2	–1.082
NR3C2	4q31.1	Nuclear receptor subfamily 3, group C, member 2	–1.099
IGF2R	6q26	Insulin-like growth factor 2 receptor	–1.011
TOP1	20q12–q13.1	Topoisomerase (DNA) I	–1.118
MS4A2	11q13	Membrane-spanning 4-domains, subfamily A, member 2	–1.175
		(Fc fragment of IgE, high affinity I, receptor for; beta polypeptide)
MRE11A	11q21	MRE11 meiotic recombination 11 homolog A (S. cerevisiae)	–1.235
COPA	1q23–q25	Coatomer protein complex, subunit alpha	–1.304
SORL1	11q23.2–q24.2	Sortilin-related receptor, L (DLR class) A repeats-containing	–1.360
NPC1	18q11–q12	Niemann-Pick disease, type C1	–1.405
FGD1	Xp11.21	FYVE, RhoGEF and PH domain containing 1 (faciogenital dysplasia)	–1.518
ABCF2	7q36	ABC, sub-family F (GCN20), member 2	–1.993
EXO1	1q42–q43	Exonuclease 1	–1.740
PLA2G1B	12q23–q24.1	Phospholipase A2, group IB (pancreas)	–2.293
Stress response
IGF2R	6q26	Insulin-like growth factor 2 receptor	–1.011
FRAP1	1p36.2	FK506 binding protein 12-rapamycin associated protein 1	–1.029
TIEG2	2p25	TGFB inducible early growth response 2	–1.082
NR3C2	4q31.1	Nuclear receptor subfamily 3, group C, member 2	–1.099
TOP1	20q12–q13.1	Topoisomerase (DNA) I	–1.118
MS4A2	11q13	Membrane-spanning 4-domains, subfamily A, member 2	–1.175
		(Fc fragment of IgE, high affinity I, receptor for; beta polypeptide)
MRE11A	11q21	MRE11 meiotic recombination 11 homolog A (S. cerevisiae)	–1.235
COPA	1q23-q25	Coatomer protein complex, subunit alpha	–1.304
SORL1	11q23.2–q24.2	Sortilin-related receptor, L(DLR class) A repeats-containing	–1.360
NPC1	18q11–q12	Niemann-Pick disease, type C1	–1.405
FGD1	Xp11.21	FYVE, RhoGEF and PH domain containing 1 (faciogenital dysplasia)	–1.518
EXO1	1q42–q43	Exonuclease 1	–1.740
ABCF2	7q36	ABC, sub-family F (GCN20), member 2	–1.993
PLA2G1B	12q23–q24.1	Phospholipase A2, group IB (pancreas)	–2.293
Transcription
IGF2R	6q26	Insulin-like growth factor 2 receptor	–1.011
FRAP1	1p36.2	FK506 binding protein 12-rapamycin associated protein 1	–1.029
TIEG2	2p25	TGFB inducible early growth response 2	–1.082
NR3C2	4q31.1	Nuclear receptor subfamily 3, group C, member 2	–1.099
MS4A2	11q13	Membrane-spanning 4-domains, subfamily A, member 2	–1.175
		(Fc fragment of IgE, high affinity I, receptor for; beta polypeptide)
TOP1	20q12–q13.1	Topoisomerase (DNA) I	–1.118
MRE11A	11q21	MRE11 meiotic recombination 11 homolog A (S. cerevisiae)	–1.235
COPA	1q23–q25	Coatomer protein complex, subunit alpha	–1.304
SORL1	11q23.2–q24.2	Sortilin-related receptor, L (DLR class) A repeats-containing	–1.360
NPC1	18q11–q12	Niemann-Pick disease, type C1	–1.405
FGD1	Xp11.21	FYVE, RhoGEF, and PH domain containing 1 (faciogenital dysplasia)	–1.518
ABCF2	7q36	ABC, sub-family F (GCN20), member 2	–1.993
EXO1	1q42–q43	Exonuclease 1	–1.740
PLA2G1B	12q23–q24.1	Phospholipase A2, group IB (pancreas)	–2.293