Colorectal Cancer
Copyright ©The Author(s) 2005. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 7, 2005; 11(33): 5156-5161
Published online Sep 7, 2005. doi: 10.3748/wjg.v11.i33.5156
Amygdalin inhibits genes related to cell cycle in SNU-C4 human colon cancer cells
Hae-Jeong Park, Seo-Hyun Yoon, Long-Shan Han, Long-Tai Zheng, Kyung-Hee Jung, Yoon-Kyung Uhm, Je-Hyun Lee, Ji-Seon Jeong, Woo-Sang Joo, Sung-Vin Yim, Joo-Ho Chung, Seon-Pyo Hong
Hae-Jeong Park, Seo-Hyun Yoon, Long-Shan Han, Long-Tai Zheng, Kyung-Hee Jung, Yoon-Kyung Uhm, Sung-Vin Yim, Joo-Ho Chung, Department of Pharmacology, Kohwang Medical Research Institute, College of Medicine, Kyung Hee University, Seoul 130-701, South Korea
Je-Hyun Lee, Ji-Seon Jeong, Woo-Sang Joo, Seon-Pyo Hong, Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, South Korea
Author contributions: All authors contributed equally to the work.
Supported by a grant of the Oriental Medicine R&D Project, Ministry of Health and Welfare, Republic of Korea, No. 03-PJ9-PG3-21600-0014 and No. 0405-OM00-0815-0001 and Korea Institute of Oriental Medicine
Correspondence to: Seon-Pyo Hong, Department of Oriental Pharmaceutical Sciences, College of Pharmacy, Kyung Hee University, Seoul 130-701, South Korea. seonhong@khu.ac.kr
Telephone: +822-961-9207 Fax: +822-966-3885
Received: March 28, 2005
Revised: April 6, 2005
Accepted: April 9, 2005
Published online: September 7, 2005
Abstract

AIM: The genes were divided into seven categories according to biological function; apoptosis-related, immune response-related, signal transduction-related, cell cycle-related, cell growth-related, stress response-related and transcription-related genes.

METHODS: We compared the gene expression profiles of SNU-C4 cells between amygdalin-treated (5 mg/mL, 24 h) and non-treated groups using cDNA microarray analysis. We selected genes downregulated in cDNA microarray and investigated mRNA levels of the genes by RT-PCR.

RESULTS: Microarray showed that amygdalin downregulated especially genes belonging to cell cycle category: exonuclease 1 (EXO1), ATP-binding cassette, sub-family F, member 2 (ABCF2), MRE11 meiotic recombination 11 homolog A (MRE11A), topoisomerase (DNA) I (TOP1), and FK506 binding protein 12-rapamycin-associated protein 1 (FRAP1). RT-PCR analysis revealed that mRNA levels of these genes were also decreased by amygdalin treatment in SNU-C4 human colon cancer cells.

CONCLUSION: These results suggest that amygdalin have an anticancer effect via downregulation of cell cycle-related genes in SNU-C4 human colon cancer cells, and might be used for therapeutic anticancer drug.

Keywords: Amygdalin, SNU-C4, cDNA microarray, Cell cycle