Dynamic expression of apoptosis-related genes during development of laboratory hepatocellular carcinoma and its relation to apoptosis

Table 1 Expression of p53, bcl-2, bax, and survivin in HCC and other liver tissues

Groups	Biopsy at	Cases	p53	Bcl-2	Bax	Survivin
			Positive (%)	Positive (%)	Positive (%)	Positive (%)
Control	30th wk	13	0 (0)	0 (0)	0 (0)	4 (30.8)
	60th wk	13	0 (0)	0 (0)	0 (0)	5 (38.4)
	90th wk	13	0 (0)	0 (0)	0 (0)	5 (38.4)
Trial	30th wk	35	0 (0)	3 (8.6)	2 (5.7)	14 (40)
	60th wk	35	21 (60)bd	7 (20)	10 (28.6)ac	13 (37.1)
	HCC	35	25 (71.4)bd	14 (40)	25 (71.4)bde	19 (57.1)

^aP < 0.05,

^bP < 0.01 vs each stage control;

^cP < 0.05,

^dP < 0.01 vs experiment for 30 wk;

^eP<0.05 vs experiment for 30 wk.

Table 2 Apoptosis indices in HCC and other liver tissues

Groups	Biopsy at	cases	Apoptosis indices
			Maximum	Minimum	Average
Control	30th wk	13	0.25	0	0.01 ± 0.02
	60th wk	13	0.25	0	0.01 ± 0.02
	90th wk	13	0.3	0	0.02 ± 0.04
Trial	30th wk	35	1.5	0	0.57 ± 0.47b
	60th wk	35	2.1	0	0.70 ± 0.60b
	HCC	35	2.5	0.1	0.71 ± 0.51b

^bP<0.01 vs same stage control.

Table 3 Relationship between expression of p53, bcl-2, bax, survivin, and cell apoptosis in HCC tissues

Observation indications			Cases	Apoptosis indices (%)			P
				Maximum	Minimum	Average
p53		+	25	2.5	0.1	0.70 ± 0.57	> 0.05
		-	10	1	0.2	0.72 ± 0.31
Bcl-2		+	14	2.5	0.4	1.02 ± 0.45	< 0.05
		-	21	1.5	0.1	0.50 ± 0.40
Bax		+	25	2.5	0.2	0.74 ± 0.25	< 0.01
		-	10	0.5	0.1	0.24 ± 0.16
Survivin		+	19	1.5	0.1	0.51±0.40	< 0.05
		-	16	2.5	0.2	0.86 ± 0.54
Bcl-2	Bax	+	14	2.5	0.5	1.02 ± 0.45	< 0.01
Bcl-2	Bax	-	10	0.5	0.1	0.25 ± 0.15