Altered profiles of nuclear matrix proteins during the differentiation of human gastric mucous adenocarcinoma MGc80-3 cells

Table 1 Nuclear matrix proteins from the 2-DE map of MGc80-3 cells identified by peptide mass fingerprinting

Number Accession Protein name	Sequence coverage (%)	Theoretical values		Experimental values		Number of matched peptides
		Mr(Da)	pI	Mr(Da)	pI
Disappeared proteins:
C33 gi|4504447 Heterogeneous	26	36 041	8.67	37 100	8.47	7
nuclear ribonucleo-
protein A2/B1
Downregulated proteins:
C19 gi|15277503 ACTB protein	37	40 536	5.55	29 000	5.51	10
C20 gi|4505773 Prohibitin	40	29 843	5.57	31 500	5.58	8
C29 gi|238427 Porin 31HL	34	30 737	8.63	34 500	8.78	7
C30 gi|4504447 Heterogeneous	43	37 429	8.97	36 600	8.85	11
nuclear ribonucleo-
protein A2/B1
C38 gi|15277503 ACTB protein	35	40 536	5.55	48 400	5.13	10
C39 gi|5030431 Vimentin	34	41 651	4.82	46 800	4.82	11
C40 gi|2119204 Vimentin	20	53 676	5.06	52 800	4.98	9
C41 gi|32189394 ATP synthase	29	56 525	5.26	55 200	5.05	15
C70 P10809 Heat shock	17	61 187	5.7	59 400	5.51	6
protein 60
Newly emerged proteins:
S78 gi|5453832 oxygen	14	111 494	5.16	92 300	5.5	12
regulated protein
Upregulated proteins:
S72 gi|15010550 heat shock	14	90 309	4.73	98 000	4.62	12
protein gp96
precursor
S74 gi|72222 heat shock	27	83 584	4.97	79 700	5.16	19
protein 90-beta
S73 gi|2134982 ubiquitin thio-	12	96 596	4.9	82 000	5.14	9
lesterase (EC 3.1.2.15)
S75 gi|6005942 valosin-	34	89 950	5.14	82 000	5.5	20
containing
protein
Unidentified proteins

Downregulated: C23, C27, C28; Upregulated: S41, S44, S53, S66, S54.