Kinetics of phytohemaglutinin-induced IFN-γ and TNF-α expression in peripheral blood mononuclear cells from patients with chronic hepatitis B after liver transplantation

Table 1 IFN-γ levels in PBMC culture supernatants at different time points

Source	DF	SS	MS	F	P
Factor (pre-/2 wk/4 wk)	2	28 770.02	14 385.01	6.946	0.027
Factor treat	2	27 072.82	13 536.409	6.536	0.031
Error	6	12 425.41	2 070.902
Treat (PHA+/–)	1	26 999.63	26 999.63	10.269	0.049
Partly factor	1	13 665.74	13 665.74	5.169	0.107

Table 2 TNF-α levels in PBMC culture supernatants at different time points

Source	DF	SS	MS	F	P
Factor (pre-/2 wk/4 wk)	1.233	32 186.69	26 113.60	22.612	0.0001
Factor treat	1.233	3 330.98	2 702.48	2.34	0.135
Error	38	27 045.63	1 154.87
Treat (PHA+/–)	1	2 363.206	2 363.21	3.192	0.09
Partly factor	1	62 804.36	62 804.36	84.833	0.0001

Table 3 HBV DNA in serum and PBMCs (mean±SD)

		IFN-γ (ng/L)	t	P	TNF-α (ng/L)	t	P
HBV DNA in PBMCs	Detected	138.08±72.44	1.065	0.297	80.75±47.30	0.407	0.686
	Undetected	164.24±72.07			74.10±49.70
HBV DNA in serum (copies/mL)	>1 000	136.77±70.04	1.702	0.097	75.37±43.02	0.402	0.69
	<1 000	175.27±71.50			81.53±52.46