Role of stress-activated MAP kinase P38 in cisplatin- and DTT-induced apoptosis of the esophageal carcinoma cell line Eca109

Table 1 Number of cells positive for p-P38 kinase upon cisplatin and DTT treatments

	D	C	D+C	U
-	26	23	15	69
±	29	31	31	21
+	24	25	24	6
++	21	21	30	4
Total scores	80.5b	82.5b	99.5b	24.5

D: DTT, C: cisplatin, D+C: DTT + cisplatin, U: untreated.

^bP<0.01 vs control group.

Table 2 SB203580 blocks DTT- and cisplatin-induced Eca109 cell apoptosis

	D	Di	C	Ci	D+C	D+Ci	U
Number of observed cells	17 925	18 889	18 162	19 220	17 929	18 260	18 430
Number of apoptosis cells	3 026	1 450	3 110	1 504	3 160	1 401	538
Apoptotic rate (%±0.00)	16.8abd	7.6b	17.1bd	7.8b	21.4bd	7.6b	2.9

D: DTT, Di: DTT-SB203580, C: cisplatin, Ci: cisplatin-SB203580, D+C: DTT+cisplatin, D+Ci: (DTT+cisplatin)-SB203580, U: untreated.

^aP<0.05 vs group treated with DTT plus cisplatin,

^bP<0.01 vs control group;

^dP<0.01 vs corresponding group in the presence of SB203580.