Screening for PreS specific binding ligands with a phage displayed peptides library

Table 1 Enrichment of phages binding to thio-PreS coupled beads

Round of panning	Thio-PreS coupled beads			Thioredoxin coupled beads
	Phage applied	Phage eluted	EF1	Phage applied	Phage eluted	EF
1st	2.0 ± 1011	2.6 ± 104	1.3 ± 10-7	2.0 ± 1011	3.4 ± 104	1.7 ± 10-7
2nd	1.0 ± 1011	1.2 ± 104	1.2 ± 10-7	1.0 ± 1011	9.5 ± 103	9.5 ± 10-8
3rd	1.0 ± 1011	9.3 ± 104	9.3 ± 10-7	1.0 ± 1011	3.9 ± 104	1.2 ± 10-7
4th	5.0 ± 1010	3.5 ± 105	7.0 ± 10-6	5.0 ± 1010	3.6 ± 104	7.2 ± 10-7
5th	5.0 ± 1010	2.6 ± 106	5.1 ± 10-5	5.0 ± 1010	8.8 ± 104	1.8 ± 10-6

¹Enrichment factor (EF) = phage eluted/phage applied.