Expression of tissue inhibitor of matrix metalloproteinases-1 during aging in rat liver

Table 1 Primer sequences and sizes of TIMP-1 and MMP-2, MMP-9 in expected PCR products for RT-PCR.

Genes	Primer sequences	Product size (bp)
TIMP-1	Sense: 5’-GCCCCAACCCACCCACAGA-3’	405
	Antisense: 5’-TTTGCAAGGGATGGCTGAACAG-3’
MMP-2	Sense: 5’-GAGAAAAGCGCAGCGGAGTGACG-3’	145
	Antisense: 5’-TTCCCCCGCAAGCCCAAGTG-3’
MMP-9	Sense: 5’-CCACCGAGCTATCCACTCAT-3’	159
	Antisense: 5’-GTCCGGTTTCAGCATGTTTT-3’
β-actin	Sense: 5’-GGCATCCTGACCCTGAAGTA-3’	565
	Antisense: 5’-GCCGATAGTGATGACCTGACC-3’

Table 2 Expression of TIMP-1 and MMP-2, MMP-9 mRNA in rat liver (mean±SD).

Age (mo)	TIMP-1/β-actin	MMP-2/β-actin	MMP-9/β-actin
3	0.29±0.08	0.52±0.04	1.48±0.14
10	0.35±0.01	0.59±0.08	1.50±0.09
24	0.89±0.28a	0.66±0.02	1.51±0.05

^aP<0.05 vs 3-mo, 10-mo rats.