Distribution of Helicobacter pylori in north China

Table 1 PCR primers for amplification of cagA, vacA and iceA sequences.

Gene and DNA region	Prime	Primer sequence (5’-3’)	Size of PCR product (bp)	References
cagA	CAGAF	GGCAATGGTGGTCCTGGAGCTAGGC	324	Pan[21]
	CAGAR	GGAAATCTTTAATCTCAGTTCGG
vacAs1 and s2	VA1-F	ATGGAAATACAACAAACACAC	259/286	Atherton[22]
	VA1-R	CTGCTTGAATGCGCCAAAC
m1a	VA3-F	GGTCAAAATGCGGTCATGG	290	Atherton[22]
	VA3-R	CCATTGGTACCTGTAGAAAC
m1b	VAm-F3	GGCCCCAATGCAGTCATGGAT	291	Atherton[23]
	VAm-R3	GCTGTTAGTGCCTAAAGAAGCAT
m2	VA4-F	GGAGCCCCAGGAAACATTG	352	Atherton[22]
	VA4-R	CATAACTAGCGCCTTGCAC
iceA1	iceA1F	GTGTTTTTAACCAAAGTATC	247	Peek[10]
	iceA1R	CTATAGCCASTYTCTTTGCA
iceA2	iceA2F	GTTGGGTATATCACAATTTAT	229/334	Peek[10]
	iceA2R	TTRCCCTATTTTCTAGTAGGT

R = A/G.

Table 2 Distribution of cagA,vacA and iceA genotypes in Zhanghe and Shenyang.

Gene	Zhuanghe area (%) n = 107	Shenyang area (%) n = 42
cagA+	101 (94.4)	42 (100.00)
cagA-	6 (5.6)	0 (0)
vacA s1	102 (95.33)b	27 (64.29)
vacA s2	0 (0)	0 (0)
vacA s-	5 (4.67)d	15 (35.71)
vacA m1a	0 (0)	0 (0)
vacA m1b	24 (22.43)	5 (11.9)
vacA m2	26 (24.3)	11 (26.19)
vacA m1b/m2	48 (44.96)	22 (52.38)
vacA m-	9 (8.41)	4 (9.52)
iceA1	10 (9.35)	1 (2.38)
iceA2	10 (9.35)	1 (2.38)
iceA1/iceA	2 84 (78.5)	32 (76.19)
iceA-	3 (2.8)	8 (19.05)

^bP<0.001 vs vacA s1 in Shenyang area.

^dP<0.001 vs vacA s- in Shenyang area.

Table 3 vacA genotypes of H pylori strains in Zhanghe and Shenyang.

Area	n	s1+
		m1b(%)	m2(%)	m1b/m2(%)	m1b-/m2(%)	S1- (%)
Zhuanghe	107	22 (20.56)b	25 (23.36)	46 (42.99)	9 (8.42)	5 (4.67)
Shenyang	42	1 (2.38)	9 (21.43)	13 (30.95)	3 (7.14)	15 (35.71)d

^bP<0.01 vs vacA s1m1b group in Shengyang.

^dP<0.001 vs vacA s- group in Zhuanghe.

Table 4 Combination genotypes of cagA,vacA and iceA in Zhuanghe and Shenyang, n (%).

cagA	vacAs	vacAm	iceA	Zhuanghe (n = 107)	Shenyang (n = 42)
cagA	s1	m1b	A1	3 (2.8)	0 (0)
cagA	s1	m1b	A2	3 (2.8)	0 (0)
cagA	s1	m1b	A1+A2	13 (12.15)	0 (0)
cagA	s1	m2	A1	3 (2.8)	0 (0)
cagA	s1	m2	A2	1 (0.935)	1 (2.38)
cagA	s1	m2	A1+A2	18 (16.82)	5 (11.9)
cagA	s1	m1b+m2	A1+A2	39 (36.45)	14 (33.3)
cagA	s	m1b+m2	A1+A2	2 (1.87)	7 (16.67)
		Others		25 (23.36)	15 (14.02)

Table 5 Multiple strains infection in Zhuanghe and Shenyang, n (%).

Genotype	Zhuanghe (n = 107)	Shenyang (n = 42)
vacA m1/m2	48 (44.86)	22 (52.38)
iceA1/iceA2	84 (78.5)	32 (76.19)
vacAm1/m2 or iceA1/iceA	92 (85.98)	33 (78.57)