CIK cells from patients with HCC possess strong cytotoxicity to multidrug-resistant cell line Bel-7402/R

Table 1 Determination of CD3CD56 of CIK cells (n = 6).

CD3+(%)				CD3+CD56+(%)
D1		D25		D1		D25
Normal	HCC	Normal	HCC	Normal	HCC	Normal	HCC
59.5±7.8a	45.1±12.1a	93.3±6.5	89.4±5.1	3.2±1.6	2.4±2.1	20.6±13.4	19.1±11.7

^aP<0.05 vs others.

Table 2 Results of cytotoxicity of CIK to target cells.

Patient Number	A value (rate of cytotoxicity)
	7402/sb(%)	7402/Rd(%)	L-02bd(%)	E control
1	0.802 (33)	0.743 (49)	1.011 (12)	0.485
2	0.913 (48)	0.895 (47)	1.201 (4)	0.627
3	0.681 (46)	0.599 (57)	0.888 (14)	0.379
4	0.630 (68)	0.602 (60)	0.959 (7)	0.401
5	0.711 (58)	0.802 (37)	1.001 (12)	0.479
6	0.987 (51)	0.903 (64)	1.302 (2)	0.718
T control	0.554	0.508	0.595

t_d = 8.70,

^bP<0.01 t_f = 8.87,

^dP<0.01.

Table 3 Average of cytotoxicity and significance of difference (n = 6).

	7402/s	7402/R
HCC	50.7	52.3
Normal	57.1	54.7

Table 4 Comparison of immune cells in immunohistochemistry.

	CD45	CD3	CD45RO	CD68
1	15.4 cells/HPF	0.15 cells/HPF	2.9 cells/HPF	8.0 cells/HPF
2	283 cells/HPF	41.3 cells/HPF	44.1 cells/HPF	77.7 cells/HPF
x-fold	18.4	275	15.2	9.7

1: before treatment. 2: after treatment. HPF: high power field.