Hydrophobicity of reactive site loop of SCCA1 affects its binding to hepatitis B virus

Table 1 Sequences of primers.

Primer	Sequence
BP1	5'- CAC AGG AGT TCC AGA TCA CAT CGA G -3'
BP2	5'- CTG GAA GAA AAA GTA CAT TTA TAT GTG GGC -3’
BP3	5'- CCG CTA GCT CAC CAT GAA TTC ACT CAG -3’
BP4	5'- CCG TCG ACT CTA CGG GGA TGA GAA TCT -3’
BPf	5'- GGA TCC ATG AAT TCA CTC AGT G -3’
BPr	5'- CTC GAG CTA CGG GGG TGA GAA TC -3’
EGf1	5'- GAT ATC ATG GTG AGC AAG GGC G -3’
EGf2	5'- GTC GAC TTA CTT GTA CAG CTC GTC C -3’

Table 2 Comparison of the relative binding to HBV in different cell lines.

Cell line	Source	HBV DNA (copies/sample)
HepG2	Human/hepatocellular carcinoma	263382±16302
WI-38	Human/lung fibroblast	186215±15236
BNL 1ME A.7R.1	Mice/liver	169221±13489
CHO	Mice/ovary	105400±25076

Table 3 Amino acid sequence differences among five SCCA1 proteins.

Amino acid residue	16	47	105	185	202	341	349	351	352	389
BP	F	D	N	Q	P	A	V	A	F	P
AJ515706	S	N	T	Q	P	A	V	A	F	S
P3	S	N	T	Q	P	A	V	A	A	S
P14	S	N	T	Q	P	R	V	A	F	S
A1	F	D	N	R	S	A	E	G	F	S