Evaluation of CMU-1 preservation solutions using an isolated perfused rat liver model

Table 1 Composition of UW and CMU-1 solutions.

Substrate (mmol/L)	UW	CMU-1
Na+	30	125
K+	125	25
H2PO4-	25	25
Lactobionate	100	100
Raffinose	30	25
Hydroxyl-ethyl-starch	50 g/L	-
Dextran-40	-	50 g/L
Histidine	-	60
Adenosine	5	-
Allopurinol	1	-
Glutathione	3	-
Insulin	100 IU/L	-
Dexamethasone	8	-
pH	7.4±0.2	7.4±0.2
Osmolarity	320 mosm/L	340 mosm/L
Viscosity	7.5 mpas	5.2 mpas

Table 2 Value of TAN and AEC after perfusate for 120 min.

Group	Time (h)	TAN	AEC
UW	6	15.14±2.13	0.56±0.04
	12	14.31±2.10	0.45±0.03
	24	13.44±1.08	0.43±0.02
CMU-1	6	16.15±2.41a	0.59±0.03g
	12	15.44±1.80c	0.51±0.01i
	24	14.54±1.89e	0.48±0.02k

^aP<0.05 CMU-1 vs UW group;

^cP<0.05 CMU-1 vs UW group;

^eP<0.05 CMU-1 vs UW group;

^gP<0.05 CMU-1 vs UW group;

ⁱP<0.05 CMU-1 vs UW group;

^kP<0.05 CMU-1 vs UW group.

Table 3 Bile flow after perfusion for 120 min (mL/kg).

Group	6 h	12 h	24 h
UW	244±12	197±3	134±5
CMU-1	249±11	201±4a	144±7c

^aP<0.05 CMU-1 vs UW group;

^cP<0.05 CMU-1 vs UW group.

Table 4 The value of ALT in the three intervals (IU/L).

Group	0	30	60	120 min
UW 6 h	17.1±1.2	65.4±2.0	88.7±1.5	321.9±2.7
12 h	28.3±3.5	80.5±1.6	122.1±2.1	408.7±5.6
24 h	52±3.0	108.7±2.5	403.7±3.1	506.7±6.3
CMU-1 6 h	17.8±2.1	66.1±1.1	87.4±1.8	319.1±5.4
12 h	31.5±1.9	79.9±2.5	125.4±2.9	419.3±6.3
4 h	48.8±1.8	110.3±2.6	398.9±4.1	510.3±4.9

Table 5 Value of LDH in the three intervals (IU/L).

Group	0	30	60	120 min
UW 6 h	228.5±5.6	378.9±4.7	499.1±10.4	805.21±1.5
12 h	380.2±4.2	480.7±6.7	600.3±11.1	900±13.1
24 h	487.5±3.8	579.6±7.3	1102±3.5	1209±12.8
CMU-1 6 h	218.5±7.4	381.6±5.7	502.4±8.6	811.3±15.6
12 h	407.2±9.1	465.7±3.9	589.7±6.9	904.6±13.9
24 h	478.5±4.3	580.7±2.9	1109±7.9	1302±14.2