Two-dimensional polyacrylamide gel electrophoresis analysis of indomethacin-treated human colon cancer cells

Table 1 Characteristics differentially expressed protein alterations of HCT116 between IN-treated and untreated groups (mean±SD).

Spot number	pI	Mr (ku)	Relative volume of spots In untreated group (%)	Relative volume of spots in IN-treated group (%)	P
1	8.611	19.869	0.181±0.019	0.106±0.024	0.04
2	7.736	22.956	0.769±0.044	0.119±0.069	0.036
3	7.619	27.827	0.238±0.043	0.136±0.041	0.043
4	7.441	38.143	0.236±0.019	0.077±0.027	0.027
5	8.079	38.304	0.319±0.029	0.122±0.035	0.034
6	7.872	40.125	0.334±0.037	0.193±0.007	0.026
7	7.946	40.232	0.117±0.007	0.020±0.009	0.002
8	6.978	39.321	0.236±0.014	0.071±0.012	0.017
9	7.166	39.536	0.233±0.023	0.070±0.014	0.01
10	7.136	48.279	0.276±0.014	0.121±0.008	0.01
11	7.719	56.079	0.227±0.040	0.065±0.027	0.006
12	7.198	56.856	0.181±0.041	0.088±0.026	0.01
13	7.692	57.936	0.314±0.031	0.146±0.023	0.044
14	7.924	58.023	0.225±0.006	0.017±0.001	0.001
15	7.163	57.003	0.166±0.005	0.017±0.001	0.002
16	5.897	52.261	0.194±0.006	0.030±0.019	0.02
17	5.648	45.818	0.415±0.024	0.110±0.009	0.004
18	5.899	47.261	0.211±0.018	0.091±0.004	0.014
19	5.471	42.589	0.150±0.088	0.058±0.008	0.011
20	5.657	39.161	0.234±0.054	0.080±0.029	0.017
21	5.913	37.714	0.209±0.038	0.094±0.042	0.005
22	5.902	35.732	0.252±0.017	0.075±0.016	0.012
23	5.894	32.625	0.278±0.057	0.104±0.035	0.021
24	5.804	34.339	0.320±0.011	0.128±0.015	0.001
25	5.665	34.768	0.212±0.030	0.057±0.015	0.042
26	5.67	32.411	0.172±0.004	0.063±0.004	0.003
27	5.749	29.912	0.100±0.011	0.005±0.002	0.014
28	5.507	32.036	0.404±0.021	0.249±0.036	0.049
29	5.605	29.737	0.529±0.076	0.309±0.047	0.038
30	5.098	25.374	0.516±0.107	0.093±0.072	0.039
31	5.044	24.428	0.293±0.009	0.077±0.016	0.002
32	3.719	18.436	0.173±0.006	0.046±0.009	0.004
33	3.943	18.306	0.239±0.021	0.049±0.009	0.005
34	5.45	21.449	0.278±0.046	0.030±0.009	0.022
35	7.019	17.438	0.137±0.003	0.446±0.023	0.006
36	7.297	28.441	0.070±0.003	0.197±0.012	0.007
37	7.104	96.008	0.094±0.005	0.186±0.008	0.02
38	3.719	40.179	0.182±0.012	0.450±0.028	0.006
39	4.185	32.786	0.343±0.042	0.512±0.063	0.029
40	4.228	30.536	0.261±0.047	0.446±0.042	0.021
41	4.274	44.732	0.055±0.004	0.193±0.012	0.004
42	3.621	85.344	0.135±0.058	0.350±0.075	0.019
43	3.665	71.952	0.133±0.034	0.342±0.032	0.01
44	7.698	38.411	0.177±0.036
45	7.752	40.5	0.121±0.032