Differential gene expression in human hepatocellular carcinoma Hep3B cells induced by apoptosis-related gene BNIPL-2

Table 1 Gene specific primers for PCR

Gene	GenBank accession number	Orientation	Sequence
BNIPL-2	AY033000	Forward	5’ CGGGATCCACCATGTACCCATACGATGTTCCAGATTACGCTCTTGGAACTATACAAGAG 3’
		Reverse	5’ CCATCGATCTATGTCCCTCCTGAGCCATGGAGATCCCGGTCCAGCTGTCTGACAGC 3’
β-actin	NM_001101	Forward	5’ AAGTACTCCGTGTGGATCGG 3’
		Reverse	5’ TCAAGTTGGGGGACAAAAAG 3’
Rad	L24564	Forward	5’ GCTGCCCTGCGAAGTGGCTC 3’
		Reverse	5’ TCCCTCCAGGGCAGGCACAC 3’
CLK-1	L29222	Forward	5’ GGGTGGTCCCAACCATGTGA 3’
		Reverse	5’ CCGGCAGAACTGTGTTCATCCCA 3’
p38 MAP kinase	L35253	Forward	5’ GTTGGAACCCCAGGGGCTGA 3’
		Reverse	5’ GGCATGTGCAAGGGCTTGGG 3’
p21	U09579	Forward	5’ CTGTGGGGGTGAGGGTCCCA 3’
		Reverse	5’ TGGGGAGGGATGGGGTGGAT 3’

Underlined sequences are restriction endonuclease recognition sites (italic) and HA-tag (bold).

Table 2 Up-regulated genes in BNIPL-2-transfected Hep3B-Tet-on cells

Location	Gene description	Induction-fold
C5n	Rad (Ras associated with diabetes)	5.886
F3e	Insulin-like growth factor binding protein 1 (IGFBP-1)	2.594
D6f	p21; Cdk-interacting proteins (Cip1); mda-6/WAF1/SDI1	2.574
E2m	Interleukin-2 receptor	2.500
D3n	Transcription factor (E2A)	2.115
F7m	Estrogen sulfotransferase (EST)	2.112
F1e	Transforming growth factor-beta (TGF-beta)	2.018
E7k	lfa-1 alpha subunit	2.012

Table 3 Down-regulated genes in BNIPL-2-transfected Hep3B-Tet-on cells

Location	Gene description	Induction-fold
B4f	Transducin beta-1 subunit 3’end	0.429
B1b	MAL protein	0.431
B5f	p38 MAP kinases/CSBP/Mxi2	0.442
A7e	Tob	0.447
A7h	CLK-1	0.457
B4b	GTP binding protein (RAB5)	0.480
A6b	p120 antigen	0.496