Association of tumor necrosis factor genetic polymorphism with chronic atrophic gastritis and gastric adenocarcinoma in Chinese Han population

Table 1 Distribution frequency of TNF alleles at 8 polymorphic loci in CAG, gastric adenocarcinoma and healthy controls

TNF locus	Allele	Size (bp)	Controls (n = 164)	CAG (n = 53)	Gastric adenocarcinoma (n = 56)	P value
TNFa	a1	97	0.0183	0.0094	0.0089
	a2	99	0.1583	0.1698	0.2054
	a3	101	0	0.0094	0.0268
	a4	103	0.0061	0.0094	0.0178
	a5	105	0.0396	0.0378	0.0089
	a6	107	0.3811	0.3585	0.3928
	a7	109	0.0793	0.0378	0.0536
	a8	111	0.0061	0.0094	0
	a9	113	0.0335	0.0566	0.0152
	a10	115	0.1189	0.1981*	0.1161	0.04
	a11	117	0.0945	0.066	0.0803
	a12	119	0.0091	0	0
	a13	121	0.0549	0.0378	0.0152
TNFb	b1	125	0.1006	0.0755	0.1339
	b2	126	0	0	0
	b3	127	0.1128	0.0943	0.0536
	b4	128	0.3293	0.3585	0.3482
	b5	129	0.4512	0.4717	0.4643
	b6	130	0	0	0
	b7	131	0.0061	0	0
TNFc	c1	159	0.2104	0.7264	0.7679
	c2	161	0.7896	0.2736	0.2321
TNFd	d1	124	0.0061	0.0094	0
	d2	126	0.0548	0.0755	0.0536
	d3	128	0.0457	0.0566	0.0446
	d4	130	0.3537	0.3774	0.3393
	d5	132	0.1341	0.1226	0.1786
	d6	134	0.3018	0.2925	0.2857
	d7	136	0	0	0
	d8	138	0.1037	0.066	0.0982
TNFe	e1	99	0.064	0.0755	0
	e3	103	0.811	0.8302	0.7768
	e4	105	0.1159	0.066	0.125
TNF-α 308	1		0.9329	0.9434	0.9732
	2		0.0671	0.0566	0.0268
TNF-β Nco1	1		0.4817	0.4528	0.4464
	2		0.5183	0.5472	0.5536
TNF- β AspH1	1		0.3079	0.3302	0.3571
	2		0.6921	0.6698	0.6429

Table 2 Association between TNFa10 allele and clinical features of CAG

Groups	Age	Gender(yr)		Atrophic degree			Intestinal metaplasia
		M	F	Mild	Moderate	Severe	Positive
TNFa10+	53.9 ± 9.6	13	8	3	12	6	14
TNFa10-	53.2 ± 12.5	19	13	6	18	8	22

Table 3 Distribution frequency of TNF TNF-β Ncol*1/2 and TNFd2/d6 genotypes in CAG, gastric adenocarcinoma and healthy controls

Group	TNF-β Ncol*1/2		TNFd2/d6
	+	-	+	-
Controls	76	88	4	160
CAG	32	21	2	51
Gastric adenocarcinoma	36a	20	6c	50

^aP < 0.05 vs compared with control group of TNF-β Ncol*1/2,

^cP < 0.05 vs compared with control group of TNFd2/d6.

Table 4 Association between d2/d6 and TNF-β Ncol*1/2 genotypes and clinical features of gastric adenocarcinoma

Group	Age(yr)	Gender		Grade of differentiation		Clinicopathologic stage
		M	F	High and moderate	Low	I-II	III-IV
d2/d6+	59.7±9.5	5	1	0	6	4	2
d2/d6-	55.1±12.5	38	12	13	37	21	29
1/2+	56.7±12.2	26	10	9	27	15	21
1/2-	53.6±11.5	17	3	4	16	10	10