NOD2 3020insC frameshift mutation is not associated with inflammatory bowel disease in Chinese patients of Han nationality

Table 1 Primers used for multiplex PCR

Primer	Sequence
Sense primer	5’-CTGAGCCTTTGTTGATGAGC-3’
Antisense primer	5’-TCTTCAACCACATCCCCATT-3’
Wild-type sense primer	5’-CAGAAGCCCTCCTGCAGGCCCT-3’
3020insC antisense primer	5’-CGCGTGTCATTCCTTTCATGGGGC-3’

Table 2 3020insC mutation in healthy controls and ulcerative colitis and Crohn’s disease in Chinese Han population

Genome	Healthycontrols(n=172)	Ulcerativecolitis(n=74)	Crohn’sdisease(n=15)
Wild type	171	72	14
Heterozygote	1	2	1
3020insC mutation homozygote	0	0	0
Wild type allele frequency	99.7%	98.7%	96.7%
Mutative allele frequency	0.3%	1.3%	3.3%