The effect of adenovirus expressing wild-type p53 on 5-fluorouracil chemosensitivity is related to p53 status in pancreatic cancer cell lines

Table 1 p53 status, transduction efficacy and IC₅₀ value of 5-FU in pancreatic cancer cell lines; mean ± SD

	Cell line	p53 status	β-gal1%	5-FU-IC50 value1μg/mL
Human
	Capan-1	159 GCC→GTC	92 (4.5)	8.25 (0.45)
	Capan-2	wild type	95 (7.2)	5.75 (0.55)
	FAMPAC	175 CGC→CAT	82 (7.8)	8.00 (0.45)
	PANCL	273 CGT→CAT	82 (7.8)	9.50 (0.75)
Murine rat
	AS	wild type	73 (5.3)	6.55 (0.45)
	DSL6A	Null	83 (6.2)	7.75 (0.55)

¹β-gal was the percentage of X-gal positive cells 24 h after AdlacZ infection at a multiplicity of infection of 50. ²IC₅₀ val-ues were defined for each cell line as the 5-FU concentration leading to 50% growth inhibtion. Results are expressed as mean ± SD from three independent experiments.

Table 2 In vitro growth of different pancreatic cancer cell lines after indicated treatment ¹measured by WST-1 cell viability assay²; % mean (95%CI)

	Cell line	Control	Ad-CD-control	Ad-p53	5-FU	Ad-p53 + 5-FU
Human
	Capan-1	97.8 (96.0-99.5)	91.9 (86.6-97.6)	55.0 (51.3-58.6)	72.9 (70.4-74.4)	30.7 (28.9-32.4)
	Capan-2	97.9 (95.7-100.0)	91.3 (88.2-94.5)	75.0 (71.3-78.6)	55.6 (53.1-58.0)	51.2 (48.0-54.4)
	FAMPAC	98.0 (95.9-100.1)	93.3 (90.5-96.2)	48.6 (44.7-52.4)	70.6 (68.0-73.1)	31.7 (29.6-33.8)
	PANCL	98.4 (97.1-99.8)	95.0 (92.2-97.8)	64.7 (59.8-69.5)	73.9 (71.6-76.2)	33.8 (31.5-36.0)
Murine rat
	AS	99.0 (97.6-100.4)	97.9 (95.7-100.0)	92.3 (88.9-95.8)	56.8 (52.2-61.3)	53.1 (48.2-58.0)
	DSL6A	98.8 (97.5-100.0)	94.0 (90.8-97.2)	64.2 (60.5-67.9)	69.4 (66.0-72.9)	32.8 (29.3-36.2)

¹Cells were either infected with Ad-CD control or Ad-p53 (MOI 1) 24 h prior ± 5FU chemotherapy (5 μg/mL) given for 48 h. ²The data represents the ratio of the colorimetric values of viable cells treated as indicated (A) compared with the untreated control cells (Ao). Results are expressed as mean with 95% confidence interval (CI) from more than 9 samples in three independent experiments.