Antitumor immunity induced by DNA vaccine encoding alpha-fetoprotein/heat shock protein 70

Table 1 Spots of IFN-γ-producing splenic cells and level of anti-AFP antibody in mice (mean ± SD)

Group	hAFP/HSP70	hAFP	HSP70	Empty	PBS
Spots ( 106 cells)	95.50 ± 10.90bd	23.60 ± 11.80f	9.25 ± 5.44	7.17 ± 4.24	5.54 ± 2.16
Anti-AFP (µg/mL)	126.50 ± 8.22bd	51.72 ± 3.40f	6.26 ± 4.27	5.83 ± 3.79	3.42 ± 2.35

^bP < 0.01, vs empty group;

^dP < 0.01, vs HSP70 group;

^fP < 0.01, vs empty group.

Table 2 Comparison of tumor growth in mice injected with hAFP-transduced SP2/0 tumor cells (mean ± SD)

Group	No. of tumor-bearing/	10 d after tumor challenge/	20 d after tumor challenge/
	No. of mice challenge	Size of tumor (mm3)	Size of tumor (mm3)
hAFP/HSP70	2/8	24.43 ± 6.10bd	37.41 ± 7.34bd
hAFP	5/8	73.64 ± 8.53f	381.13 ± 15.48f
HSP70	8/8	118.24 ± 14.65	785.83 ± 13.87
Empty	8/8	132.26 ± 17.27	817.51 ± 16.25
PBS	8/8	149.73 ± 16.54	860.53 ± 14.72

^bP < 0.01, vs empty group;

^dP < 0.01, vs HSP70 group;

^fP < 0.01, vs empty group.