Effects of platelet-derived growth factor and interleukin-10 on Fas/Fas-ligand and Bcl-2/Bax mRNA expression in rat hepatic stellate cells in vitro

Table 1 Primer sequences for PCR and amplification conditions for each target gene

Primer (base)	Sequence	Amplification conditions
Fas 414	5’-GAATGCAAGGGACTGATAGC-3’	Denaturation at 94 °C for 45 s,
	5’-TGGTTCGTGTGCAAGGCTC-3’	Annealing at 55 °C for 30 s and synthesizing
		at 72 °C for 1 min for 25 cycles
FasL 239	5’-GGAATGGGAAGACACATATGGAACTGC-3’	Denaturation at 94 °C for 45 s,
	5’-CATATCTGGCCAGTAGTGCAGTAATTC-3’	Annealing at 55 °C for 30 s and synthesizing
		at 72 °C for 1 min for 33 cycles
Bcl-2525	5’-TATGATAACCGGGAGATCGTGATC-3’	Denaturation at 94 °C for 45 s,
	5’-GTGCAGATGCCGGTTCAGGTACTC-3’	Annealing at 60 °C for 30 s and synthesizing
		at 72 °C for 1 min for 33 cycles
Bax 310	5’-GACACCTGAGCTGACCTTGG-3’	Denaturation at 94 °C for 45 s,
	5’-GAGGAAGTCCAGTGTCCAGC-3’	Annealing at 60 °C for 30 s and synthesizing
		at 72 °C for 1 min for 30 cycles
β-actin 660	5’-CCAACCGTGAAAAGATGACC-3’	Changed according to different target genes
	5’-CAGGAGGAGCAATGATCTTG-3’

All initial denaturations were at 94 °C for 5 min. Finally an additional extension step at 72 °C for 7 min was done.