Gene distribution of cagII in Helicobacter pylori-infected patients of Zhejiang Province

Table 1 PCR primers for amplification of cagT, ORF13 and ORF10

Gene	Strand	Primer sequence	Length (bp)
cagT	+	5’ TCTAAAAAGATTACGCTCATAGGCG 3’	490
	-	5’ CTTTGGCTTGCATGTTCAAGTTGCC 3’
ORF13	+	5’ CGTTCATGTTCCATACATCTTTGGC 3’	617
	-	5’ GATTTATAGCGATCTAAGAAACCGC 3’
ORF10	+	5’ AATAGTGCTTTCTTTAGGATTAGCG3’	658
	-	5’ CCGATTTAATCCTTTCGCTTATGTG 3’

Table 2 Relationship between cagT, ORF13, ORF10 gene expression and clinical diagnosis in patients of Zhejiang Province

Group	n	cagT n	%	ORF13 n	%	ORF10 n	%
Chronic superficial gastritis	70	67	95.7	66	94.3	69	98.6
Chronic atrophic gastritis	31	31	100.0	29	93.5	31	100.0
Gastric ulcer	41	39	95.1	39	95.1	41	100.0
Duodenal ulcer	21	21	100.0	21	100.0	21	100.0
Both gastric and duodenal ulcer	3	3	-	3	-	3	-
Gastric adenocarcinoma	5	5	-	4	-	5	-
Total	171	166	97.1	162	94.7	170	99.4