Characterization and enrichment of hepatic progenitor cells in adult rat liver

Table 1 First and second antibodies for immunohistochemistry

First antibody	Dilution	Second antibofy	Dilution	Chromogen
OV6 (mouse IgG)1	1:100	Goat anti-mouse IgG (HRP	1:150	DAB (brown)
		or FITC)	1:200	green fluorescence
CK19 (mouse IgG)2	1:150	Goat anti-mouse IgG (HRP)	1:150	DAB (brown)
Albumin (rabbit IgG)	1:400	Goat anti-rabbit IgG (AP)	1:50	Fuchsine (red)
				or NBT/BCIP (blue)
AFP (mouse IgG)	1:400	Goat anti-mouse IgG (HRP)	1:200	DAB (brown)
Connexin 43 (mouse IgM)2	1:400	Goat anti-mouse IgM (HRP)	1:200	DAB (brown)
C-kit (rabbit IgG)3	1:50	Goat anti-rabbit IgG (AP)	1:50	Fuchsine (red)
				or NBT/BCIP (blue)
BrdU (mouse IgG)	1:50	Goat anti-mouse IgG (HRP)	1:150	DAB (brown)

Note:1 = a gift from professor Sell S, 2 = from Sigma, 3 = from Santa Cruz, others were from DAKO Company.