Loss of heterozygosity on hromosome 1 in sporadic colorectal carcinoma

Table 1 LOH frequency of 21 microsatellite markers on chromosome 1

Locus	Location	LOH case	Normal case	LOH rate (%)	Informative rate (%)
D1S468	1p36.33-36.31	19	33	36.54	62.65
D1S450	1p36.31	7	41	14.58	57.83
D1S2697	1p36.22-36.13	5	25	16.67	36.14
D1S255	1p34.1-32.3	11	43	20.37	65.06
D1S2797	1p32.3	8	46	14.81	65.06
D1S2890	1p32.3-32.1	6	48	11.11	65.06
D1S2841	1p31.2	11	46	19.30	68.67
D1S207	1p31.2-22.3	5	63	7.35	81.93
D1S2868	1p22.1	10	39	20.41	59.04
D1S206	1p22.1-21.2	7	33	17.50	48.19
D1S2726	1p13.3-13.1	6	48	11.11	65.06
D1S498	1q12-21.2	11	48	18.64	71.08
D1S484	1q22	10	55	15.38	78.31
D1S218	1q24.1-24.3	16	42	27.59	69.88
D1S238	1q31.1	3	38	7.32	49.40
D1S413	1q31.3	13	27	32.50	48.19
D1S249	1q32.2	10	36	21.74	55.42
D1S213	1q41-42.12	8	41	16.33	59.04
D1S2800	1q42.12-42.2	12	45	21.05	68.67
D1S2785	1q43	10	48	17.24	69.88
D1S2836	1q43-44	12	36	25.00	57.83

Table 2 Relationship between clinicopathological features and LOH of 11 loci on short arm of chromosome 1

		D1S468		D1S450		D1S2697		D1S255		D1S2797		D1S2890		D1S2841		D1S207		D1S2868		D1S206		D1S2726
		N	L	N	L	N	L	N	L	N	L	N	L	N	L	N	L	N	L	N	L	N	L
Gender	Male	18	8	20	4	13	2	19	7	20	5	21	4	20	6	31	2	15	3	17	3	19	5
	Female	15	11	21	3	12	3	24	4	26	3	27	2	26	5	32	3	24	7	16	4	29	1
Age (yr)	> 60	26	13	30	7	19	4	32	8	36	7	37	6	34	11	46	5	32	7	27	5	33	6
	≤ 60	7	6	11	0	6	1	11	3	10	1	11	0	12	0	17	0	7	3	6	2	15	0
Location	Proximal colon	12	9	12	2	13	2	14	5	19	2	22	3	21	4	24	2	13	6	11	1	20	0
	Distal Colon	7	5	10	1	4	2	12	2	8	3	11	1	9	4	17	1	13	2	8	4	13	0
	Rectum	14	5	19	4	8	1	17	4	19	3	15	2	16	3	22	2	13	2	14	2	15	61
Gross pattern	Massive	15	9	16	2	10	1	15	7	21	3	17	5	20	4	23	2	17	3	17	1	19	2
	Ulcerative	13	5	16	3	9	3	22	4	19	3	20	1	18	4	27	3	15	3	10	3	20	3
	Encroaching	5	5	9	2	6	1	6	0	6	2	11	0	8	3	13	0	7	4	6	3	9	1
Size	≥ 5 (cm)	18	8	21	5	13	1	19	8	24	4	27	2	23	4	31	1	19	5	13	5	24	3
	< 5 (cm)	15	11	20	2	12	4	24	3	22	4	21	4	23	7	32	4	20	5	20	2	24	3
LN metastasis	LN (+)	24	10	26	5	17	4	29	4	28	6	31	5	26	9	38	5	22	8	20	5	28	4
	LN (-)	9	9	15	2	8	1	14	7	18	2	17	1	20	2	25	0	17	2	13	2	20	2
Differentiation	Well	8	7	14	1	5	1	10	3	20	1	15	0	12	4	16	2	13	2	14	3	18	0
	Moderately	16	10	19	5	12	4	21	5	14	7	19	5	22	5	31	3	16	5	13	2	18	6
	Poorly	3	0	4	1	2	0	5	0	4	0	5	0	2	0	6	0	3	1	0	2	5	0
	Mucinous	6	2	4	0	6	0	7	3	8	0	9	1	10	2	10	0	7	2	6	0	7	0
Dukes stage	A	3	3	4	0	1	0	4	1	3	2	4	0	7	0	7	0	5	0	2	1	5	0
	B	6	6	11	2	7	1	10	6	15	0	13	1	13	2	18	0	12	2	11	1	15	2
	C	17	8	20	3	11	2	21	3	20	5	23	5	18	7	26	5	16	7	18	3	20	4
	D	7	2	6	2	6	2	8	1	8	1	8	0	8	2	12	0	6	1	2	2	8	0

¹P = 0.002, LOH frequency of rectal cancer vs colon cancer.

Table 3 Relationship between clinicopathological features and LOH of 10 loci on long arm of chromosome 1

		D1S498		D1S484		D1S218		D1S238		D1S413		D1S249		D1S213		D1S2800		D1S2785		D1S2836
		N	L	N	L	N	L	N	L	N	L	N	L	N	L	N	L	N	L	N	L
Gender	Male	25	6	25	7	22	6	20	1	14	7	16	6	17	3	19	9	25	3	19	5
	Female	23	5	30	3	20	10	18	2	13	6	20	4	24	5	26	3	23	7	17	7
Age (yr)	> 60	37	10	43	6	30	14	28	3	18	10	28	7	33	6	33	10	39	10	26	9
	≤ 60	11	1	12	4	12	2	10	0	9	3	8	3	8	2	12	2	9	0	10	3
Location	Proximal colon	14	6	20	4	16	5	15	1	10	6	17	3	15	2	15	6	18	6	16	5
	Distal colon	14	3	14	4	14	4	10	1	7	3	4	2	12	3	14	1	14	2	6	5
	Rectum	20	2	21	2	12	7	13	1	10	4	15	5	14	3	16	5	16	2	14	2
Gross pattern	Massive	22	4	23	3	18	11	12	2	14	5	13	4	17	4	17	3	20	6	16	5
	Ulcerative	17	5	24	3	15	4	19	1	9	5	13	5	19	4	18	6	20	3	12	5
	Encroaching	9	2	8	4	9	1	7	0	4	3	10	1	5	0	10	3	8	1	8	2
Size	≥ 5 (cm)	21	7	27	7	22	6	18	3	11	6	16	5	21	4	21	8	23	7	17	8
	< 5 (cm)	27	4	28	3	20	10	20	0	16	7	20	5	20	4	24	4	25	3	19	4
LN Metastasis	LN (+)	31	6	36	4	26	11	23	2	16	6	22	6	28	5	30	7	31	7	25	5
	LN (-)	17	5	19	6	16	5	15	1	11	7	14	4	13	3	15	5	17	3	11	7
Differentiation	Well	12	4	15	3	11	3	8	1	7	3	10	2	13	2	13	5	16	2	10	5
	Moderately	26	4	27	4	22	7	19	0	12	6	17	4	18	5	21	5	18	7	18	6
	Poorly	2	0	6	0	3	2	3	0	2	1	2	3	4	0	4	1	4	0	2	1
	Mucinous	8	3	7	3	6	4	8	2	6	3	7	1	6	1	7	1	10	1	6	0
Dukes stage	A	4	0	5	2	3	2	5	1	3	2	2	1	3	1	4	0	4	1	2	3
	B	13	5	14	4	13	3	10	0	8	5	12	3	10	2	11	5	13	2	9	4
	C	22	5	26	4	18	9	18	1	12	4	15	5	21	5	21	6	23	6	17	2
	D	9	1	10	0	8	2	5	1	4	2	7	1	7	0	9	1	8	1	8	3