Liver Cancer
Copyright ©The Author(s) 2003.
World J Gastroenterol. Jul 15, 2003; 9(7): 1455-1459
Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1455
Figure 1
Figure 1 Recombinant plasmid pGEX-4T-1(His)6C-ARL was identified by restrictive endonuclease and polymerase chain reaction (1. 5% agarose electrophoresis). Lane 1: The recombinant plasmid pGEX-4T-1(His)6C-ARL digested by EcoR I only, about 5.9 kb fragment could be seen; Lane 2: The recombinant plasmid pGEX-4T-1(His)6 C-ARL digested by EcoR I and Xho I, two fragments of 5.0 kb and 950 bp could be seen; Lane 3: pGEX-4T-1(His)6C-ARL being template, 950 bp was amplified by polymerase chain reaction; Lane 4: positive control of PCR; Lane 5: negative control of PCR; Lane 6: GeneRulerTM 100 bp DNA ladder plus.