Abstracts Open Access
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 82-82
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.82
Clinicopathological study in treatment of chronic hepatitis with hyperbaric oxygenation
Wei Liu, Wei Zhao, Chan Luo, Department of Pathology, the Second Hospital of Nanjing, Nanjing 210001, Jiangsu Province, China
Xiang Lü, Department of Pathology, Nanjing Drum Tower Hospital, The Affiliated Hospital of Medical College of Nanjing University, Nanjing 210008, Jiangsu Province, China
Xiao-Gang Zheng, Department of Pathology, General Hospital of Nanjing Military Region, Nanjing 210000, Jiangsu Province, China
Author contributions: All authors contributed equally to the work.
Supported by The Natural Science Fund of Nanjing, No. 95821
Correspondence to: Dr. Xiang Lü, Pathological Department of Nanjing Drum Tower Hospital, Zhongshan Road 321, Nanjing 210008, Jiangsu Province, China. glyyjszx@publicl.ptt.js.cn
Telephone: 25-3304616-662 Fax: 25-3317016
Received: April 3, 2000
Revised: May 10, 2000
Accepted: June 18, 2000
Published online: September 15, 2000

Abstract

AIM: To probe into the feasibility and theoretic basis for the treatment of chronic hepatitis with hyperbaric oxygenation (HBO).

METHODS: Sixty cases of chronic hepatitis were randomly distributed into an experimental group (n = 30) and a control group (n = 30). The experimental group was treated with HBO for 6 courses. The control group was treated with commonly used drugs in clinic for 60 d. The function and blood stream graph of liver were examined and the liver biopsies were made before and after treatment. The routine paraffin slides of liver tissue were cut, stained with HE, and observed under optical microscope. The ultrathin slides from paraformaldehyde and glutaraldehyde fixed liver tissue were cut, stained with lead citrate, and observed under transmission electric microscope. The HBsAg and HBcAg in the experimental group were detected by the ABC immunohistochemical method before and after treatment.

RESULTS: In the experimental group the ALT, SB, γ-GT, AKP, IgG and IgM in blood (P < 0.05) and the degeneration and necrosis of hepatocytes (P < 0.05) were remarkably decreased, the mean contractive wave of blood stream in the liver (P < 0.05) and the blood stream in the right ramus of janitrix (P < 0.05) were significantly increased, and the swelling of mitochondria, the increased lysosomes, the generation of Kupffer’s cells, the infiltration of lymphocytes in portal area and the capillary generation were remarkably alleviated (P < 0.05), but the fibrosis and fat storing cells did not reduce (P > 0.05) in the liver, and the expression of HBsAg and HBcAg in the liver was not lowered (P < 0.05) after the treatment with HBO.

CONCLUSION: The treatment with HBO for chronic hepatitis was effective and recommendable.

Key Words: Hepatitis/therapy, Hyperbaric oxygenation, Liver cirrhosis, Hepatitis/pathology, Liver/ultrastructure, Immunohistochemistry



Footnotes

E- Editor: Zhang FF

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