Abstracts Open Access
Copyright ©The Author(s) 2000. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 15, 2000; 6(Suppl3): 104-104
Published online Sep 15, 2000. doi: 10.3748/wjg.v6.iSuppl3.104
Effect of glutathione on gastric mucosal lesion induced by restraint Water-immersion in rats
Jun-Li Wan, Chang-Liu Wang, Section of Physiology, Department of Biological Science and Technology, Yantai Teachers College, Yantai 264025, Shandong Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Fund of Educational Committee of Shandong Province. No. J94k39
Correspondence to: Jun-Li Wan, Section of Physiology, Department of Biological Science and Technology, Yantai Teachers College, Yantai 264025, Shandong Province, China. wanzhang@public.ytptt.sd.cn
Telephone: 535-6672619
Received: November 9, 1999
Revised: June 3, 2000
Accepted: July 10, 2000
Published online: September 15, 2000

Abstract

AIM: To determine the effect of glutathione (GSH) on stress gastric mucosal lesion.

METHODS: The stress gastric mucosal lesion as produced by restraint water-immersion in rats and gastric mucosal lesion, gastric mucosal GSH content, gastric acid secretion and gastric barrier mucus secretion were examined. We also observed the effect of GSH on gastric mucosal lesion and the effect of N-ethylmaleimide (NEM) and indomethacin on GSH protection. Comparisons between two groups were made using the Student’s t test.

RESULTS: GSH (100 and 200 mg/kg) intraperitoneally protected against stress gastric mucosal lesion (P < 0.001 and P < 0.001). Restraint water immersion stress significantly reduced gastric mucosal GSH content (P < 0.001), but pretreatment with GSH (100 mg/kg) had no effect on gastric mucosal GSH content (P > 0.05). The preinjection of NEM (10 mg/kg. sc.), a sulfhydryl blocking reagent, or indomethacin (5 mg/kg, im.), a cyclooxygenase inhibitor, had no effect on protection of GSH (P > 0.05). GSH (100 mg/kg) significantly increased secretion of gastric barrier mucus (P < 0.05), but had no effect on secretion of gastric acid in restraint water immersed rats (P > 0.05).

CONCLUSION: GSH can inhibit the formation of gastric mucosal lesions induced by restraint water-immersion. The protective effect of GSH was due, in part, to promoting the secretion of gastric barrier mucus, but not to suppress the gastric acid secretion. The protection effect of GSH has no relation with gastric mucosal GSH and PGs.

Key Words: Gastric mucosal lesion, Glutathione, Stress, Gastric acid, Gastric mucus, Rats

Footnotes

E- Editor: Hu S

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