Original Articles
Copyright ©The Author(s) 1999.
World J Gastroenterol. Dec 15, 1999; 5(6): 465-469
Published online Dec 15, 1999. doi: 10.3748/wjg.v5.i6.465
Figure 1
Figure 1 A Western blot of purified conjugates. One milligram of LLO and the final conjugate either with or without DDT (100 mM) redu ction were run on a 7.5% SDS-PAGE gel. The proteins were transferred to anylon membrane, quenched, and probed with a polyclonal antibody to LLO. Detection of the antigen-antibody complex was determined by exposure to anti-rabbit IgG ho rseradish peroxidase and developed with 3’,3’-diaminobenzidine and hydrogen peroxide as described in Materials and Methods. Molecular weight markers, lane 1; LLO alone, lane 2; ASOR-PL, lane 3; ASOR-PL-LLO-DNA, lane 4; ASOR-PL-LLO-DNA + 100 mM DTT,lane 5.