A DNA delivery system containing listeriolysin O results in enhanced hepatocyte-directed gene expression

Figure 1 A Western blot of purified conjugates. One milligram of LLO and the final conjugate either with or without DDT (100 mM) redu ction were run on a 7.5% SDS-PAGE gel. The proteins were transferred to anylon membrane, quenched, and probed with a polyclonal antibody to LLO. Detection of the antigen-antibody complex was determined by exposure to anti-rabbit IgG ho rseradish peroxidase and developed with 3’,3’-diaminobenzidine and hydrogen peroxide as described in Materials and Methods. Molecular weight markers, lane 1; LLO alone, lane 2; ASOR-PL, lane 3; ASOR-PL-LLO-DNA, lane 4; ASOR-PL-LLO-DNA + 100 mM DTT,lane 5.