Published online Oct 15, 1998. doi: 10.3748/wjg.v4.iSuppl2.81
Revised: June 22, 1998
Accepted: July 17, 1998
Published online: October 15, 1998
AIM: To establish a quick and easy method for detecting the ant i-HAV IgM level in sera of HAV patients during acute periods.
METHODS: To use micro-blot immuno-binding assay (MBIMA) was used to detect the titration of Goat-anti-Human(GaH) IgM-HRP and titration of IgM in sera of healthy people. RIA method and MBIBA methods were used to detect the IgM level in sera in 114 HAV patients and 48 non-HAV patients.
RESULTS: Titration of IgM and GaH IgM-HRP in healthy people were 1:1000 and 1:1600, respectively; The IgM level in sera in 114 patients was positive by RIA method and that in 112 patients was positive by MBIBA method; the IgM level in sera in 48 non-HAV people was negative by both methods; the duration of RIA and MBIBA were 2-d and 6-h, respectively.
CONCLUSION: The positive and negative coincidence rates of RIA and MBIBA in detecting the serum IgM level in patients were 98.24% and 100%, respectively; the MBIBA method was much easier and quicker than RIA and might be used widely in clinical practice.
- Citation: Wang RH. Early diagnosis of hepatitis A virus-micro-blotting immuno-binding assay. World J Gastroenterol 1998; 4(Suppl2): 81-81
- URL: https://www.wjgnet.com/1007-9327/full/v4/iSuppl2/81.htm
- DOI: https://dx.doi.org/10.3748/wjg.v4.iSuppl2.81
E- Editor: Li RF