Published online Oct 15, 1998. doi: 10.3748/wjg.v4.iSuppl2.64
Revised: September 14, 1998
Accepted: September 29, 1998
Published online: October 15, 1998
AIM: To study the effects of tumor necrosis factor (TNF) on proliferation cycle of human gastric cancer cell.
METHODS: Exponentially dividing cells were collected. Cells were treated with TNF at 234 U/mL, harvested at every 24 h up to the 96 h, stained with propiodium iodide, and analyzed for cell cycle distribution by flow cytometry. Percentages of cells in the different cell cycle phase were calculated.
RESULTS: The proliferation index (PI) of SGC-7901 droped from 1 9.8% to 5.6% in 72 h (P < 0.01). When SGC-7901 cells were exposed to TNF for 72 h, the proportion of G-2M DNA content increased from 6.5% to 49.8% (P < 0.01). However, the proportion of G0, G1 and S phase DNA contents decreased from 73.7% to 44.6% (P < 0.01) and 19.8% to 5.6% (P < 0.01). The effects of TNF reduced 72 h later.
CONCLUSION: TNF can inhibite SGC-7901 cell proliferation and arrest cells in G-2M. The peak period is 72 h.
- Citation: Huang G, Zhang SB, Cai ZQ, Chen L. Cell cycle-specific effects of tumor necrosis factor on human gastric cancer cell line. World J Gastroenterol 1998; 4(Suppl2): 64-64
- URL: https://www.wjgnet.com/1007-9327/full/v4/iSuppl2/64.htm
- DOI: https://dx.doi.org/10.3748/wjg.v4.iSuppl2.64
E- Editor: Li RF