Exploring the regulatory mechanism of tRNA-derived fragments 36 in acute pancreatitis based on small RNA sequencing and experiments

Figure 2 Effects of tRNA-derived fragments 36 on acute pancreatitis progression in MCP-83 cells. A: Gene expression of tRNA-derived fragments 36 using reverse transcription quantitative polymerase chain reaction; B-D: The expression levels of inflammatory factors in the acute pancreatitis (AP) cell model, including interleukin-1β (B), interleukin-6 (C), and tumor necrosis factor-α (D); E: The expression levels of lipase in the AP cell model; F: The expression levels of amylase in the AP cell model; G: Cell viability examination using Cell Counting Kit-8 assays; H: Cell death examination using terminal deoxynucleotidyl transferase dUTP nick-end labeling analysis. Error bars represent the mean ± SD. a, b, and c represent different expression levels, the same letter represents no significant difference between groups (P > 0.05), different letters represent significant difference between groups (P < 0.05). tRF-36: tRNA-derived fragments 36; IL: Interleukin; TNF: Tumor necrosis factor; AMS: Amylase; NC: Normal control; DAPI: 4’,6-diamidino-2-phenylindole.