Basic Study
Copyright ©The Author(s) 2020.
World J Gastroenterol. May 28, 2020; 26(20): 2584-2598
Published online May 28, 2020. doi: 10.3748/wjg.v26.i20.2584
Figure 1
Figure 1 Schematic summary of the 3 amplification steps. In the first amplification step (PCR1), a large region was amplified. In the following step (PCR2), the region was divided into 2 amplicons that overlapped in a 112 nucleotide-long portion. In the third step (PCR 3) a sample identifier (MID) was added. PCR: Polymerase chain reaction; MID: Multiplex identifier.