Abnormal expression of HMGB-3 is significantly associated with malignant transformation of hepatocytes

Figure 1 Dynamic upregulation of HMGB3 in rat hepatocarcinogenesis. Rat hepatocarcinogenesis models were successfully made by consistent 2-AAF intake. A: The dynamic alterations of liver morphology (upper panel) and H&E staining (lower panel) of liver tissues in rat hepatocarcinogenesis. The livers of the rat model, according to the results of rat liver H&E staining, were divided into normal, degeneration, precancerous, and HCC group. B-E: the dynamic alterations of the HMGB family at the mRNA level in models were detected by RT-qPCR. B: HMGB1 mRNA. C: HMGB2 mRNA. D: HMGB3 mRNA. E: HMGB4 mRNA. Each band was presented as a relative value normalized to normal controls (n = 6). F: the immunohistochemical staining of rat HMGB3 expression in different groups. ^aP < 0.05. 2-AAF: 2-acetylaminofluorene; H&E: Hematoxylin and eosin; HMGB: High mobility group-box; RT-qPCR: Reverse transcription-quantitative polymerase chain reaction.