Generation of glyceraldehyde-derived advanced glycation end-products in pancreatic cancer cells and the potential of tumor promotion

Figure 4 Western blotting analysis of cleaved caspase-3. PANC-1 cell lysates (15 μg of protein/lane) and Jurkat cell lysates (10 μg of protein/lane) were loaded on a 40-150 g/L polyacrylamide gradient gel. Proteins on the PVDF membrane were probed with anti-cleaved caspase-3 and anti-GA-3 phosphate dehydrogenase (anti-GAPDH) antibodies. U: The lysate of Jurkat cells not treated with cytochrome c. T: The lysate of Jurkat cells treated with cytochrome c. Western blotting was performed for three independent experiments. GAPDH was used as a loading control. GA: Glyceraldehyde.