Three-dimensional perfused human in vitro model of non-alcoholic fatty liver disease

Figure 3 Hepatocytes cultured in fat media have altered gene expression profiles. Hepatocytes were cultured in fat or lean conditions for 7 d before total RNA was extracted and gene expression was compared using Fatty Liver RT2 Profiler PCR Arrays. A: Gene expression changes were defined by a fold change > 1.95 and P ≤ 0.05; B: Fold change in expression in fat vs lean condition of key genes, filled bars = fat, white bars = lean. Data are mean ± SD from nine independent cultures (three donors per condition and n = 3 per donor).