Published online Jan 14, 2017. doi: 10.3748/wjg.v23.i2.204
Peer-review started: August 17, 2016
First decision: October 11, 2016
Revised: October 25, 2016
Accepted: November 16, 2016
Article in press: November 16, 2016
Published online: January 14, 2017
Core tip: We report the development of an in vitro, fully human, three-dimensional cell culture model of non-alcoholic fatty liver disease (NAFLD). The model recapitulates key features of clinical NAFLD, with primary human hepatocytes continuously loaded with fat, for up to 14 d of culture, without causing any hepatotoxicity. Fat loading caused transcriptomic, proteomic and metabolic changes to the hepatocytes, including reduced activity of CYP3A4 and CYP2C9 enzymes. Fat loading in the model could be modulated using known anti-steatotic drugs (e.g., metformin), demonstrating the utility of the model for screening the efficacy of novel anti-steatotic compounds.