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Copyright ©The Author(s) 2016.
World J Gastroenterol. Feb 21, 2016; 22(7): 2219-2241
Published online Feb 21, 2016. doi: 10.3748/wjg.v22.i7.2219
Table 1 Perturbations in the intestinal microbiotain irritable bowel syndrome patients
Ref.MethodsN° of Pts.Diagnostic criteriaResults in IBS in comparison with healthy subjects
Dlugosz et al[38]qPCR (small bowel)35Rome IINo differences
Balsari et al[39]↓Coliforms
↓ Bifidobacteria
Si et al[40]Culture25Rome II↓Bifidobacteria
Malinen et al[41]qPCR27Rome IIVeillonellain C-IBS
Lactobacillus in D-IBS
Mättö et al[42]Culture/DGGE26Rome II↑ Aerobes
Temporal instability
Swidsinski et al[43]FISH20A-IBS↑ Mucosal bacteria
D-IBS↑Clostridium coccoides
Maukonen et al[44]PCR-DGGE16A-IBSHigher instability of the bacterial population
C-IBS↑Clostridial groups
Kassinen et al[45]16S ribosomal RNA gene cloning24Rome IISignificant differences in several bacterial genera belonging to the genera Coprococcus, Collinsella and Coprobacillus
Lyra et al[46]16S ribosomal RNA gene cloning20A-IBS C-IBS D-IBSClostridium thermosuccinogenes in D-IBS ↑Ruminococcus torques in D-IBS ↑Ruminococcus bromii-like in C-IBS
Krogius-Kurikka et al[47]16S ribosomal RNA gene cloning10Rome IIProteobacteria and Firmicutes in D-IBS
Actinobacteria and Bacteroidetes in D-IBS
Kerckhoffs et al[48]FISH, PCR41A-IBS↓Bifidobacteria
Carroll et al[49]16S ribosomal RNA gene cloning2Rome III↑Bacteroidetes↑Proteobacteria
Salonen et al[50] Review
Tana et al[51]Culture26Rome II↑Lactobacillus
qPCR↑ Veillonella
Carroll et al[52]qPCR10Rome III↓Aerobicbacteria
Codling et al[53]16S rRNA-DGGE47Rome II↓Bacterial richness
Ponnusamy et al[54]rRNA-specific 16S rRNA-DGGE54Rome IISame total bacterial quantity Higher diversity of Bacteroidetes and Lactobacilli
PCRLower diversity of Bifidobacter and Clostridium coccoides
Kerckhoffs et al[55]16S rRNA-DGGE37Rome II↑Pseudomonas aeruginosa
Rajilić-Stojanović et al[56]16S rRNA62Rome II↑Ratio of the Firmicutes to Bacteroidetes
qPCRDorea, Ruminococcus, Clostridium spp
Bacteroidetes, Bifidobacterium, Faecalibacterium spp
Carroll et al[57]16S rRNA16Rome III D-IBSLower biodiversity of microbes
Carroll et al[58]16S rRNA23D-IBS↓Bacterial richness
Parkes et al[59]FISH47Rome IIIMore total bacteria numbers
Bacteroides, Clostridia coccoides-Eubacteriumrectale
Jeffery et al[60]16S rRNA37Rome IIA sub-group of IBS showed normal-like microbiota
A sub-group of IBS showed large microbiota-wide changes with ↑Firmicutes and ↓Bacteroidetes
Chassard et al[61]FISH/16S rDNA14Rome IIEnterobacteriaceae
Functional approachesRome III↑Suphate-reducing bacteria
C-IBS↓Lactic acid bacteria population (bifidobacteria and to a lesser extent, lactobacilli)
König et al[62] Review
Sundin et al[63]16s rRNA19Rome IIIBacteroidetes in the PI-IBS group (13 patients) ↑Firmicutes (more specifically Clostridium in IBS)