Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Nov 21, 2015; 21(43): 12361-12369
Published online Nov 21, 2015. doi: 10.3748/wjg.v21.i43.12361
Figure 3
Figure 3 Hepatitis C virus nonstructural protein 5A modulates phosphorylation levels of key insulin signaling molecules. Untransfected and nonstructural protein 5A (NS5A) transfected cells were incubated with insulin (100 nmol/L) for 3 h. An equal amount of cellular lysates were subjected to Western blot assay using anti-p-Akt Ser473, anti-p-Akt Thr308 and anti-p-IRS-1 Ser307. Cellular actin was used as an internal control to verify protein loading in each lane. IRS: Insulin receptor substrate.