IGF2 differentially methylated region hypomethylation in relation to pathological and molecular features of serrated lesions

Table 4 Clinicopathological and molecular features of fifteen traditional serrated adenomas with high-grade dysplasia

No.	Age/sex	Location	Size (mm)	KRAS mutation	BRAF mutation	PIK3CA mutation	MGMT methylation	MLH1 methylation	MSI status	LINE-1 methylation level	IGF2 DMR0 methylation level	IGF2 expression
1	75/M	Rectum	8	c.35G>A (p.G12D)	Wild	Wild	(-)	(-)	MSS/MSI-low	58	70	Weak
2	54/F	Sigmoid colon	20	c.35G>A (p.G12D)	Wild	Wild	(+)	(-)	MSS/MSI-low	53.7	39.5	Strong
3	62/F	Transverse colon	15	c.38G>A (p.G13D)	Wild	Wild	(+)	(-)	MSS/MSI-low	53.3	72.0	No expression
4	84/M	Rectum	5	c.35G>A (p.G12D)	Wild	Wild	(-)	(-)	MSS/MSI-low	65.0	26.5	Moderate
5	85/M	Sigmoid colon	12	Wild	c.1799T>A (p.V600E)	Wild	(-)	(-)	MSS/MSI-low	58.0	45.5	Strong
6	48/M	Sigmoid colon	20	Wild	c.1799T>A (p.V600E)	Wild	(-)	(-)	MSS/MSI-low	53.7	40.5	Moderate
7	69/M	Sigmoid colon	10	Wild	c.1799T>A (p.V600E)	Wild	(-)	(-)	MSS/MSI-low	58.7	52.0	No expression
8	60/M	Descending colon	9	Wild	c.1799T>A (p.V600E)	Wild	(-)	(-)	MSS/MSI-low	59.0	41.5	Moderate
9	34/M	Sigmoid colon	18	Wild	c.1799T>A (p.V600E)	Wild	(+)	(-)	MSS/MSI-low	57.3	42.0	Strong
10	61/M	Rectum	10	Wild	c.1799T>A (p.V600E)	Wild	(-)	(-)	MSS/MSI-low	56.7	29.0	Strong
11	52/F	Ascending colon	15	Wild	c.1799T>A (p.V600E)	Wild	(+)	(+)	MSS/MSI-low	57.0	57.0	Moderate
12	70/F	Rectum	13	Wild	c.1799T>A (p.V600E)	Wild	(+)	(+)	MSS/MSI-low	63.0	84.5	Weak
13	66/F	Ascending colon	12	Wild	Wild	c.1624G>A (p.E542K)	(-)	(-)	MSS/MSI-low	49.7	28.0	Moderate
14	52/M	Sigmoid colon	12	Wild	Wild	Wild	(-)	(-)	MSS/MSI-low	48.0	44.5	Moderate
15	69/F	Rectum	13	Wild	Wild	Wild	(+)	(-)	MSS/MSI-low	44.3	80.0	Weak

HGD: High-grade dysplasia; MSI: Microsatellite instability; MSS: Microsatellite stable; TSA: Traditional serrated adenoma.