Original Article
Copyright ©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 28, 2012; 18(16): 1892-1902
Published online Apr 28, 2012. doi: 10.3748/wjg.v18.i16.1892
Figure 3
Figure 3 The specific expression of tetracycline reverse transcriptional activator in the livers of albumin-tetracycline reverse transcriptional activatortrangenic mice and in-alb-urokinase plasminogen activator transgenic mice. A, B: Reverse transcription polymerase chain reaction (A) and Western blotting (B) analysis of tetracycline reverse transcriptional activator (rtTA) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression in different tissues of the 6-8 wk old F1 albumin-rtTA PCR-negative (left for A, B) or positive (right for A, B) transgenic mice. 1: Liver; 2: Brain; 3: Thymus; 4: Heart; 5: Lung; 6: Kidney; 7: Spleen; C: Western blotting analysis of rtTA and GADPH expression in the liver extracts of mice with different genotypes.1: In-alb-urokinase plasminogen activator (uPA) mice group; 2: Liver extracts of wild type mice group; 3: tetO-uPA mice group; 4: Albumin-rtTA mice group. pTet-on transfected Huh7 cell extracts were used as positive control (+).