Brief Article
Copyright ©2011 Baishideng Publishing Group Co.
World J Gastroenterol. Nov 28, 2011; 17(44): 4883-4889
Published online Nov 28, 2011. doi: 10.3748/wjg.v17.i44.4883
Figure 1
Figure 1 Sensitivity of recombinant acyl-CoA synthetase 5 proteins to triacsin C. Enzyme activity of Ni2+-affinity purified recombinant r-ACSL1, r-ACSL5, hu-ACSL5 C-terminal 6xHis-tagged enzyme, and hu-ACSL5 N-terminal 6xHis-tagged enzyme were assayed at pH 7.5 (white symbols) and pH 9.5 (black symbols) in the presence and absence of triacsin C as described in the methods section. ACSL5: Acyl-CoA synthetase 5.