Original Article
Copyright ©2010 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 28, 2010; 16(32): 4047-4054
Published online Aug 28, 2010. doi: 10.3748/wjg.v16.i32.4047
Figure 1
Figure 1 Levels of ErbB2 expression in three cholangiocarcinoma cell lines and inhibition of ErbB2 phosphorylation by AG825. A: Cells cultured in 10% fetal bovine serum (FBS)-containing medium were lysed and the lysates were analyzed for ErbB2 mRNA by real-time reverse-transcriptase polymerase chain reaction. Data are presented as mean ± SE of ErbB2 mRNA level normalized with β-actin mRNA obtained from three independent experiments; B: Cells were incubated without and with 30 μmol/L AG825 in 10% FBS medium for 6 h, then lysed and analyzed for ErbB2, phospho-ErbB2 (Y1248) and β-actin by Western blotting. The results shown are representative of three separate experiments. bP < 0.01 vs HuCCA-1 and KKU-100.