Effect of oxymatrine on the replication cycle of hepatitis B virus in vitro

doi:10.3748/wjg.v16.i16.2028

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Apr 28, 2010 (publication date) through Apr 23, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 28, 2010; 16(16): 2028-2037
Published online Apr 28, 2010. doi: 10.3748/wjg.v16.i16.2028

Table 1 Sequences of primers and probes for PCR or RT-PCR

Primer set			Taqman MGB probe			Target
Name	Sequence	Position¹	Name	Sequence	Position¹	Target
CCP1	5’TTCTCATCTGCCGGACCG 3’	1562-1579	TCC	5’FAM-CCTAATCATCTCTTGTTCAT-MGB 3’	1836-1855	cccDNA
CCP2	5’CACAGCTTGGAGGCTTGAAC 3’	1883-1864	TCC	5’FAM-CCTAATCATCTCTTGTTCAT-MGB 3’	1836-1855	cccDNA
RCP1	5’TCCTCTTCATCCTGCTGCTATG 3’	404-425	TRC	5’FAM-TGTTGGTTCTTCTGGACTA-MGB 3’	437-455	rcDNA
RCP2	5’CGTGCTGGTAGTTGATGTTCCT 3’	510-489	TRC	5’FAM-TGTTGGTTCTTCTGGACTA-MGB 3’	437-455	rcDNA
PGP1	5’CTCAATCTCGGGAATCTCAATGT 3’	2429-2451	TPG	5’FAM-CCTTGGACTCATAAGG-MGB 3’	2459-2474	pgRNA
PGP2	5’TGGATAAAACCTAGCAGGCATAAT 3’	2659-2636	TPG	5’FAM-CCTTGGACTCATAAGG-MGB 3’	2459-2474	pgRNA
BAP1	5’ACGGCCAGGTCATCACCAT 3’	2396-2415	TBA	5’FAM-CAATGAGCGGTTCCG-MGB 3’	2418-2432	β-actin
BAP2	5’AGGCTGGAAGAGTGCCTCAG 3’	2438-2457	TBA	5’FAM-CAATGAGCGGTTCCG-MGB 3’	2418-2432	β-actin

¹Nucleotide position in U95551 HBV sequence or M10277 β-actin gene sequence. HBV: Hepatitis B virus; cccDNA: Covalently closed circular DNA; rcDNA: Relaxed circular DNA; pgRNA: Pregenomic RNA; MGB: Minor grooving binder; PCR: Polymerase chain reaction; RT-PCR: Reverse transcription polymerase chain reaction.

Citation: Xu WS, Zhao KK, Miao XH, Ni W, Cai X, Zhang RQ, Wang JX. Effect of oxymatrine on the replication cycle of hepatitis B virus in vitro. World J Gastroenterol 2010; 16(16): 2028-2037
URL: https://www.wjgnet.com/1007-9327/full/v16/i16/2028.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i16.2028